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CRIMIC CR02161

Gene tow

CG14821

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:6942856..6949313	65D3	FBti0213474	91455

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GGAGAGCAACGTGTCGAGCAACAAAT	ATGGCTCCTCACATGTTGTTCG

Images

Construct Design Sequences

sgRNA sequence	CATAAACGACCCAAATATTTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACGGTGCACCAGACGTAAAT	ACAATGGCGCCTTTGTGTATG	CGGAGGCGCCTAAGCTTTAG	TCACTAAGTGGACTCTCACCTC

 

Amplicon 5'

Amplicon 3'

ACGGTGCACCAGACGTAAATGCGTTAAAGATGAAGCAAATCAATAGGTTATCTAAGGGTTTTCATAAGCCCAAAATACACAGTTCAAATATTTTTAGTAGCCACCTATGTGCATATATATAATAAAAAATCAGCAATACTGATAAATTGATCTTCAAAAATTTCTCAGTTCATTTTACTTATAGATTTTATGTTATTCTCATTTTGCGTGCTAGAATTGATATTGTTTTGCCAACTTTGTGTATGCCCGATCATTTGGAATGATCTATCAAATCGTTTAGGCATTTTCCTCTGTGTAGAGCAAGGGAAATTGGGGAAACGGCGAGGAAAACGTATTGCCTCGCACAACAAAAGCTTACACGCATGACGACATCAATTTGCCTCCAGCTGTGTGTGCATGTGTGCGTCCGTGTTTCCGTGCATGTGTGTGTGTGTGAGCCACCTGTTTCCACACAATGAGGACATCATTGATTAATATGTCACCAGCAGTGAAAAAAGAAGGGGAAGTTGAAAGGAGGAGCCCAGTTTTAAGGCCATACATTCACCAAAGTAATAATAAAGGCCTACATTTTAAAGCCCACAGCATTGTCTATATATAAACACACACACACGCACACTAGCCAATTTAAGTTTATAAGAATGTAAAAACCTAGCCTACATGGCAAACTTTTGACTATGCCCAACCCCGCGCGTTTGTTGCTGTCTGTGTCTATGTGTGCGTGTTTCTTGCCTTTTTTATTTCATTTTGTGTGTGTATAATTAACTGCTAAAAAGGCAACTTGTTGTTGCAGTGGGCGCCGCTTCAAGGGGCGTGGCCAGCCCCCCCCCCCCAGTTTGTATATAGCTGTGTGTGTGTGTGTCTCTGTGGGCAAAAGGGTTAAAGCTAAAAAAGTATAGCATACACAAAGGCGCCATTGT

CGGAGGCGCCTAAGCTTTAGTCTCGTTCCTAGATTAATTATTCATAATACTCGCGGCTCTCGCTTTACCGAATTTGCATTAAAATGCATAACGGCTTTTAATTAAATTTATGGCTGGCAAGGCCACTTCAAAGAAAGGCCCGAGGAAATATTAAGTATACGCAGGCTAAGCCGAAAGATGGATTAGCAAAACAGAAACGTGCAGGTACAAAAAGGATGCTAAAAACTTTGATTTCTTTATTTATTTTTATAAATAATTAAGTATTTGAAAGGCAGATAGTGTTTTTTTGAGTATCCATAGTGGCCCAGGACCTAGCATAATTTCTACAAGTGTTTTCTAAGCATTTTTCTCGACTGCGACTGCCCCCCGTTTCGCAGAGAAGTTTGCCACACTTGGCTTATTGCAAAACTTTTGCCACTCCATGGCACTCGCGAAATTGCCCACTCATCTTGACTAATGCTCGAAACTCTTCTTTGGCATTATCCATTTGTTTGGCAGCCGACTCAGATGCCGAAACGGACGAGGACGAAGGCCATATCGAGGATGCTGAGAAGGCACAGCAGCGCGACCGCGAGAAGCACGACGAGAGCGAGGAGCTAAGCAATAAGGACATCCAGGAAAGCGACGAGGACGTGGCCGTCTCACTGCTGCGCGCCAAGAACCTATCGCTGCTGCAAAGCCAGTAAGTTAGCCCATCGACCGCATAACCCGAAAATGAACACCTCACTTTTCCAGGGAAATATCATCTAGCCAGCAGAACTTCTTCCGCATGCTGGACAAGAAGATTGATGAGGTGAGAGTCCACTTAGTGA

 

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