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CRIMIC CR02162

Gene CG17189

CG17189

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:27101230..27102917	97E2	FBti0213475	91456

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTTCGTTCGTGCCATAGTTGTCAGC	CTGGATACTGTTTCCCGATCCCATAC

Images

Construct Design Sequences

sgRNA sequence	AGGTCGTTGAGCTCCTCTATAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGGTTGCAAGGTAAAGTCGC	GAGGAGCTCAACGACCTCAA	GGCAAACATCTCTGATGGGA	GCACGAGGGCGAATATCTCA

 

Amplicon 5'

Amplicon 3'

CGGTTGCAAGGTAAAGTCGCTACAGTTTCAGAGTCGCATAACAACAGCAATGATAGTAGCTACACAGCGAGAAATGCTGCTCATAAAGAATATTTATGTATTTATTTCTTCGATCCGGCAAAACATTTTTATACGCACTCTATTTACTACTAAGTTAAATTACCCTAAATATGAATTGGAAATAAAATTTTTATATACTTTTTATATACTTTCTTAAAAAAAAACCTATTGATAATTTTTCATTATGTGATTTTTCTGTACTCTCGTGCAATAAATGTGGAATAAATTTAATTTTGCATTGCGAGCGAATGAAAATGGATTGATTCTGGTATTACCCAGAGACTCCAGATTCAATTCATCAATTGCAGTCGCCGAGTCATCAGCAGCGGCTATATATATTGTCCAGATAAAAACTAGCGCCACTACGAGTTGTATCTCATTCGCAGCTCGTGCGTAGTTAACTCGATTAAGGATCTCCGCTTGTTGTGTCTGCGATTCCCGCCGTGACAGTGACGCAAGCTTTCCACCATGCTCCTAATTGGATTACTAATGCTACTCCATGCCGGCCTCCAAGGAGCCCAGTGTGTTGCCTTCTACACGGAAAAACGTAAGTCCACAAAAGGACTTTAAAATTATGAGGAAATGGAAAATGCTGAACAATTTGAAAGAGCTATCAAAAGTGAACAAAATACTGTATATAATAATCGATATGATTTCATTGTAGCCTCCTATATTGAGTCCTGCAAGATTTATGAACCGGAGTTCACCAAGTGCTCAACTCGTTCAATACAGGCATTTATGAACCAGCTCGTCAAAGGTGAGATATCACCATGATAACCACGACTTGTGCGACTGACTTCCTTTGTTATCCTTTAGGTGTGCCCGAAATAGAGGAATCCTTTGGACCCATCGATCCCATGAGGCAGGAACAGCTGGTGTTCAAGCAGGATAACAGCGACGTGGCCACCCTTTCCGCCAATCTTACGGATATGCTGATCAGGGGCTTTGGCAAAATGCTGATCAAGGAGAGCAAGTGAGTAGCTATCGATTCCACATAGCCGTCAAGAGCAATCAAAAGTTGCTCCATTGAGGTCGTTGAGCTCCTC

GGCAAACATCTCTGATGGGAAACACCCGGACATAAATTTGTTCATACCAATTTAATAGCAAAACGCGATTTAGACTATTTAATATTATTTCACATTGATCTATCAACTAGTTTCGCGCACATGGTATTGTCAGTCTTAATAAATTGGCTATTAAATGAGCTGGCTTATAGGAGTAAAGCATTTGTATCTTTATTTGCCCACCAGAGTCAGCAAAAAGGATTTCAGCTGGCTAACTAAGATCTACCTGCCCCAAATGCGAATAGATGGTCACTACAAAATGGTCGGTCGCATTTTGCTGGTGCCTCTGCAGGGAAATGGAAAAATAGTCATGGAAATAGGTAGTTGCTAATGATAATTGTTACTAAATGAATGTAAACCGGATTTTTTGCCACTGACAGATGATCTGGACATACTGATGACCACCAAAACACGCCTCTACGAGAAGGGCGGCTATACATTTTACAACGTGACATCCGTGAAGGTAAAAGTGGACGTCGGCAAAGTTCGCACCAGATTGGATAACCTCTTCAACGGGCACAGCAAGGAGGTGGAAGATAGCACCAATCAGTTCTTTAACGACAATTGGAAGGATGTCTTCGAGGCACTGCGTCCGCTGGTGGTTGAAACCGTGGAAAGAACGCTACTAGATCTACTCCACAAGACATTTGCCCTGTTTCCGGCCAGCTTCTTTGTGGAGGACATACCCACCTCCTTAACCTTGTATGGTCGCAAGTCACACATGATCACTTGAAAGGTGTTCCATTGAAATTTAATCCCACTTCCTTCAAACTGCCTTGGAATCTGCATCTTCTCTATGAAAATCTTACTGAAATTAATATAGAAATATTTCGAGGCAATCAAAACAATTTGATAGTTCTAAGAACTTTTTTCATTTGTTCATTGCTGTCTTTTGATGAGTTTAGATTTTTGTTTTTAACGCTTTTATTATCTGTGAGTGCCTAGACTAAATGTTAGTGTAACTTATTAAATGCTTAGTTTAGAGGTATCCTAATATGGGATGGCACAGTGCTCCACATCCGGCTGCCAGTTCGAGTCGATAGATCAGTAGAACCAGTCGTACTTGAGATATTCGCCCTCGTGC

 

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