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CRIMIC CR02163

Gene CG31345

CG31345

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:12811145..12817029	87D1	FBti0213476	91457

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAACGGGTTGGACAAGGATCCAATT	ATAAGCGTCGTTATCGATCGAGGC

Images

Construct Design Sequences

sgRNA sequence	GGGCTGAGTCAAACACTGTGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGTTACGATGCCATCCGGAT	GTTTGACTCAGCCCACTGTT	GGATTGTGGAAAGAGGGCGT	GCTTAGGTGAGGCTGACTGT

 

Amplicon 5'

Amplicon 3'

GGTTACGATGCCATCCGGATTTGGAGCACCCACCTCGAAGTTTTTCAGGAACTGCGTGAATATCTGGTTTTCCGTCATCTCGCCACTTAAGTATTTCGGATGATCGCGCACCGAATATACATTCTTCAAATCGGTGACAGTAATTTGGCCATCGCCATTGGCATCCATCTTATCGAAGGCCTTTTCGATGATACTGATACGCGAATTATTCATAGGAGGCTGAAAACAAAGATATTCTCATAAAAACATTGTTTTGCATTAAATAAACATTTTATTTACACGCAGCTTTACTAGGAATTCTGTCATATTAATATTGCCACTTCCATCTGTGTCGAATCTGGGAAATAAATTAAAAATTGTATTTAATATCATATTTTATTTTTTGTATTTTTACAATGAATTATGATGATTTTTATGTTTCAAGAGTTATTTAAAAACTTTTTTCGTTCAACTACTAATTGAATAAGAGCTCAAGTCTCCATTTTGCTTTTAAGTTGATAATTACCAACCTGGAGAACATTTCATCGATTTCGGAATCAGTCAGGTCCAGGCCGATATCCGTGACTCCTTTTTTGAACTCCTCGGGACTGAGAGATTTGCTGCCATCGTCGTCCATCACCCGGAATGATCTGGAAATATAAAACGAAGTCAGAAACGAGAGGCGCACAAAAATAATTCGCAATATGAATGGCAAAATGAAAATATATTCCTATTATTGCCACGGGACAAAGCCAGCCGGATAAGAATATGCCATTGATAGATACTCAGAAGAGTGGGCGCCAGTGGGATCGGGAGTGGGAGCTCATTCAGCTATGCATTCGATGCATTGAATTTTCCGGCTGGCAAACAAACAAATTGCATAAATACAAATGAAAATGCGCGCTCACGAGGACGAGGCCAATAGGCACAGCCACCATCCGACCGACAAGCCAACAAACAAACAAATGCACATACCGTACATATGTTGCAGATGCGAGGATTTTGTACAAAATGTCTATATATTCAAAACGGGAAATTGTTAGCAGACAGCCGGGCGGGAAAAACAGGTGGGGCTGGAGATTTTGGGCGGTGGGCGGTACAGGTGGACGCGGTTTAGCTCTACTCTGCTCTCAGCTCAAATTGAAATAAAAACTAACAGTGGGCTGAGTCAAAC

GGATTGTGGAAAGAGGGCGTGGCAGGTCGCTGAACTGGAAGAAAAAGTAGTTTCTATACGAAAAAAAATATTGTTTTACATCTTTAGTGCACTTATTTAAATCTAATTTTTAATGTGTTGAAATCAGTTCAATAAATAAATTCATTTAATTTGATTGTGGGATTCCAGGCTCTTTAATTTCTTTAAGTGCGGCTTTGCTCGACAGCAACTACAGAAATCCAATTATGTTTGTTTGCATTCAGTCAGCGATTTTTGCCACTCCCCCTTCGGCATCCTACTCCTCAGTCTTCCAGCGAGCTTCATAGTTATAGTTTTTTCTATTTTTCCCTTTTTTCCTGCCCGGCCTGCACACACTTGAAAGCGCTTTCGAGAATGTTAGTTCAACATGCACTCGAACTCTCGGTATGCTCGTATTTACATGCGCCTAGCTGAGCCCAAACTTAACATCATCATCAGGAGCAGCGGTAGCAGAAAAAGCCGGCAAAGTTTCAGTTTCATTTGCCATATTTCCTACCCAGCTCCGACCATCTTCACCTGCCCTTTTTCTGGCTATATGTTCTCTATTCCGTCACTTGTGGGACCCTCAACGATTTCATCATTTTAATACTGCCCCTCGGGGATGCAACGCTACACAAGAGGCTCATCTGCATCTCAGGCGAATAATGGGGGAATTCTGCTCACATCTCTTGCACATCTCCACATTTCGTATTTTGCATGTTGCCAAAAAAGTTTGATAAACCTTGCACACTTTAGGAATCCAAAAGTGGCGAATATTCACGATTTATAAATTTATACCTAACCAATTCTAGCAAATAACAAAATTGTATTTAAAAACAAAGGCGTTTCAATGAAGTATATAATGAATACATATTACTCCCTTAGCGAGAAATAGTCCCACTTTGATACTAATGACAAAGCTAATTAAATATTTGATCAACTCCATACAGTTTTAAGAGAATTACCGCTTCGTTTACCGAAAGTGTATGCAGATAAGAAACAAATAGCAAACAATCCTCCGTATGAAGCCTGCTGTATCTGAAACACACACAACTGGAGCACCATTATGCAAACAGTCAGCCTCACCTAAGC

 

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