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CRIMIC CR02164

Gene MBD-like

CG8208

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:9559760..9561533	85D24	FBti0213477	91458

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCCAGCAGCTCGTAGATGATCACGAA	GAACTCATGTCTTGAGTGCATCCTGC

Images

Construct Design Sequences

sgRNA sequence	TCGTGTGCGTGTGTATACATAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATGTTGTCACCGCGGTAGTT	CATAGGCGTATGCGTGTGT	CACACGCACACGAACTACCA	GCTTCTCCCAGAACAGCTGT

 

Amplicon 5'

Amplicon 3'

ATGTTGTCACCGCGGTAGTTCCGCGATATCAGCACCTTGCCCTTCACGTCCAGAACGAAAATGGCCGACGAAGACATGTCTCTTGGTTCCCAGCAGCTGCCACTTGTCCCAGGTGTAAAATTCCCAATTGGATCGAGTGGGAGGTCGCTTCGATGGTTCTACTGCTCGCGGCAGGCGTTGCGCGTGGAAGTTGGCAACTGTCGCGAGGTGGCGATGAGTGGGCTAGGTCTATCAATGGGCTGGATTTACATATTCCGTTTCGTAGACTGGAAAATGCGAATGAAAACTCCGGAATTTCTTACGTCGCCTTTTCTGCTGCTTCTTTTTCTTATTAGTTGGCCCACCAGTGCTGTGAAATGTTCACATCAGGTGTTGGGCAGTGTGGCCAGGTGAGATTGGTCTACTGCTGATTAACTAGCGAATTAAGCTTAAAGGAAATGAAAAATGTGCAAGGTGTAACATCTTAAGGTTTCTGTTTTTAAATAAATACCTTTTAAATTATTTTCATTTTCCCATTGGTACCTTTAAATTCAAATCATAAATATATTGGAGAATGTAATCTAGCATCACTATTGTGCCATCCGGCATTTGTGGCCATCCGCAGTACGGCCACACTGCTAGCCCAGACGCTGCTTTTGTGGAATTGTTAATTATTTATTTCGGCGAGCATGCAAATGAACCCGAGCGTCACAATCGAGCGCAAACGCGTCGACTGCAGTGTCCTGCCAAAGGGATGGCAGCGCGACGAGGTCCGAAAGTCCGGCAGCAGCGCCAATAACAACGCCAGCAGCAACAACAACAGCAGTGCCACAGCCAGCAGCAACAACAACAATAACAAGGTGGATGTTTTCTACTACAGGTAAAGAAGAAGAGCAGAATTACTATATAGACTACTATATACCTAAAATCGGAACACTGCACGCTGTTTAATCGGATAAACGTTTATAATCACTTTCACTACCTTTCTGTTATTACATCCGCATAACCACACACACACACACCATATACACCACACACACGCATACGCCTATG

CACACGCACACGAACTACCACCTAACCCACCAAAAATATCCATCCACCGCCGTGTCTGTTCAGCAGTCCCACAGGAAAAAGGGCGGAGGGAAAGCCACAGGACATTGCAATCCCTGATTTCCAGCCTGGCAAAATGCCGCACTGCGCCCTGCCCTCGCCCTCCATTTCGCTGTACCGCTGCAGCGCCATGCCGCTGCCTATAGCAAGCGGGGGCGGTAATGGGGCCACCAGCGGATCGGCGGCCAATGCCCTCAAGCGCAAGTTCGCCCGGAGTCAAGGAGGCAATGCTGCAGGAGCAGCAGGAGCTGCTCCTCCGGCAGCGACCGCCTCGTCAGCAGCAACAGCGACAGCAGCATCTGCTTCACCATCAACGGCCAATCGCCAGCAGCAACAGATCGAACTCAGGTAGAGCCAAGTGGATGAGCACTTGACTCTTGCATGTCAGCGTGTCATGTCTTGTTTGCCAGGACGCAAGGTCTACAGCCTGCGGCAATTAAAAAGCATCAAATGAAAAAATACCCAAAGAAACACACAATTGGCAAAACAATTCGTAGTTGTCTTCAAAAACGCTGTAGCTCTGTGCCACCTGAGATTTATGTTCCACCATCTCTGCATGCTAAAGCTTTACTTTACGCTTTGTCTAATGTGCTGTTCGGTGTTTTAGCTTTTTGAAGGCGTTTCTCTAATACATTTCCAATCTTTAATCCCTTACAGCCGAGCCCTGCGCACTGATGTCTCCCTGGTGCCTCCCATTCGACAGACTGCCTCGATCTTCAAGCAACCGGTGACGGTGATTCGCAACCATAAACAGGATCCCGCCAAAGCGAAGAATGAACCCAAGCATGGCACTCGGGAGAAGCCGAAACAGCTGTTCTGGGAGAAGC

 

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