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CRIMIC CR02171

Gene CG17270

CG17270

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:20816445..20818929	93A1	FBti0213479	91460

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACGAATCTCTCGAGTGGGTTGTGCAA	GCTGTGAGTTTTCGTCATCCAGATCC

Images

Construct Design Sequences

sgRNA sequence	CCACGTGGTCGCGTGCAGCCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGCGATATGGTGTAGTCCCC	CGGGAATTACGCTCCGCATA	TGGAGCGCTTATGGTGGATC	TGTGCTGAAGTCCATGGACC

 

Amplicon 5'

Amplicon 3'

CGCGATATGGTGTAGTCCCCTGTGAAAGAACCCATCAAATGCGACTCCAGCAGCTCAATCCATCCCCACTTACCCTCTATGAACTGGGTGTCGCTGTGATCGCCCAGGAAGCTGCGTCTTTCTATGTAGCTCCTAAAGGCCGTGCCCAGGTCCATGAAGGCCACGAATGCTATCCATCCCCGGAAGGCGTACAGCAATCGCTCGCGGGGTCGTCCGCCAGACTGCATGGCAGCCCTACAAGTAAATTTTCTGTGTTATTTAATATCCATTCTCCCTTTAAAGGAAAATAATTTAATCTAAGGAATGGGTTTACCAATTGGTCTAATGTCCAACACAACTTGAAGACTTTATTACACATATCAGGGTTATATATTTAGAAAATTATTTGAAATTTTGACAAAATGGTCATTATACTGCAGAAAACTTGTAATAGAATGTACAATGTAACAATTCGTCGTAAGGTTTTTTTATGCCTACTCGTATTCGTGTAACGAATGTTTTAAGATATAAGAAAAACTCATTAAGAACCTTAGCCAGAAAATATACAAATTTAGCTCAGGTCATAGTATCATTATGCTTAAAGCAGACCGACCCAGTCTAGATTATGATGATATAATAAGAGAACCAACTATGTACATATTTCGGTATTACAAAGCCATTAGAATAAACGCGATACTATTGCGTGTGCATAATGAGGTGCTTTTGTTTAAAGGCTTTTAGCACAAGCATGAACTCCAAGCGAGCAGCCGAGTCCAATGGCATCGGTGGCAACCGGCTTATCTTCCCCTGAGAATCGCATCGCACTCGGAAGTCCCAGCACATGCCACCACCCACCCCACTCCACTTCACTCCGCAGGTCTGGGCAAAATCGATATTATGGCACACTATGCTGCTGTGCTATTTGCGCAATGCAAATATGCGGAGCGTAATTCCCG

TGGAGCGCTTATGGTGGATCCACCATTAGTCCCGGTTGTTTATTTCCAAGCAACATCGCCGACTGGGTCGTCCGAGCAACGCGATTTATGGGCCCCGGACCAACCGGCTAGCCACTATCAGGCGGTTCACAATAAATGCACCACTGGCTATGTGTGTATTTGATTTCGGGAGCACTCCTCAATTCACTCACATTTTGCAAAGATTCCCGTGTTAATCAGCAGTACCGCGATCCGTCCCACCCGGCGTATGCGTAATATTTGTTTGCAGCACTGCCACTGGCACTCTTGTGTCCCTCAAAAACCTATTTTATGCAGCAGCGATTGTTTATTTCATATATGTGTATTTTCGCCGTTCAGCTGTAAACGCGACCGATTCTCGTACCGCCGCCTGCAACGCCTTGCCAGCCACTAAAAGTCTAGAATGGGAAAGTTTCGTGCGAGGACAGGCAGCTTTTATGCTTTCGGAGCTTTGGTTCTTGTTGATTGGTTCGTATCGCACCGGGAATACCTGGAAAAAGTGGTCTAAGTCCTCAATTAATATAGTATGATTATGATTAAAAAAAATATATTCACAAGTTGGCCATTACTGACTTAAAAAAAAAGTGTGGCTTGCATTTTTAGCATCATTTTATTTAGCAAAGTACAAAAGTTGTAAACTGTGTTCAATCGGAATTGCTATATGTATAGGCAACATGTATAAAAGATCAACAATTTGTCAAGTTCTATGGCTCTCTTGGGATGAAACTGGTCTAGTCTGGTCTAGTCCTTGCCAATGTTGGCCTCAGTCTTGCGGTACTCGGCCCAGTCGACAAATCCATCGTTGTTCAGGTCCATGGACTTCAGCACA

 

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