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CRIMIC CR02172

Gene CG7896

CG7896

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:29983426..29990785	99D1	FBti0213480	91461

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATTTGCCCATGGACCGCAGAGTAA	ACCGAAGAAGTTCTCGGACAGATCCA

Images

Construct Design Sequences

sgRNA sequence	CGACAGCGAATTTCCGCCGTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGATCATGGCTCACCGAACC	CGCTTTTGCTATGGTGACCAC	CGTCGGTCGGGCATTATT	GGCACCGATGTCAGGCTATT

 

Amplicon 5'

Amplicon 3'

AGATCATGGCTCACCGAACCGCTCTTGCGCTTCAAAGGCGTTTTGTATAGGAACTTTACAGTCGAACTTCAGCAAGTGATTATGCATTCAGTTGTGGTTTTTATGAAGTGATGTACTGATGGGTTGATCTTATTTAATATCGTAAATAATTTATCATATATAAGACAAAAGGCAGGAGATAAATCTTTTCCACAGATACTCATTCACTCGTAAATTGTATTTATCAAAGAACATTTATCAAAGAACTTTGAGATACAAATTCCTTGCGGTTGGATTATGATCAACAAGTAGAATTTCATTATCTTCTAGATTTTAGATTTTGGTAGCTGAGATGTGTTCGCTTATAGAAAGGCTCTACTGTCTGCTCTAGTAATTAACGCTTACTTATCAAGCATAAACTGGAATTTCGCTGATGGCCTAAAAGAAATATATTCATGAATTGAATTGAATGAGCGTAGTAATGTTTCATAGAGTTACTTTTAGCAGATACATGGCATATAGACAGCAAAACACATTAGATTCACACCTTCGATGTTTAGAATGACGAATAAGACAACAACAAATGCCTCTAATTTGTCCAAGTTCACCAAAATTACTCATACCACTCGTGGGCAATCGTGGCTCTTTGTTGGCCAGATCGCTTGGATTATAGCCTTCGTTCTTTCGATCGGACCTTGGGTATTGGACCATTGTTGTTGGGCGCAAGTATGCGGCTTGTATCGAAATAGCCAGAGATGCCGGAGATCGGTATGCAAATCAAATGTTCTAAGCCAACGCGGCGATAATGGCCAAATGACCAAATACGATGCGGAATAGAGATCTCCTCTCGAAACGAGTTTCTATCAGCGCGGCCAAATCCGTTCGGGCCAGAAACCCAGTAATAATAGTGTCATTCCTCAATTGCAGCGACATGGAAGCGTGGAAACAGCTGCCAAACGGAGGGCGTTTCCTGCTGCTTCTCCTGGCCTGCATCGCCCACCTGGGCCACCCAATCCTCGCCTGGCGCCCCTGTCCGGAGCTCAGTCCCGCCCTCCGTCTGCCATGCAGGTGAGTTTCATAGCACTTAGACCACAGTATGCCATTCACTCAGTGGTCACCATAGCAAAAGCG

CGTCGGTCGGGCATTATTTTAATATCACTAAATTACCGCCAACTGCAATTTCATTTGGCTTTGTTTTTCTGCAATCACCTGCCACGGCCTGACGACAATTACAGGTGCAATGTGGTTCCGTTTGCGGCGACTGGCCAACTGGGAGCCGTGGCCATGGACTGCGATCGCGTTGTCTTCCACGGCGATGCCCCGCAACTGCCTTATGGAGCGCCCATCGTGGCGTATACACAACGTCACAGCGGCCAACAGGTGCTGCCCGCCCAGGTGAGTGTCGCAATCGAATGGAGATAAGCTGTGAAGCTGTGAAGAGATCGGGGATTTCGACACTCTTCGATGATTTCACAGGCACAATAAATGCCCCCAAATGGAGGTCAATTAGATCTTATTTCTTACCGATTTGACTCATATGTATATGCTGTCGAAATGTTACTTTTCCATTCGTTTAAGATCATATTTCTATAATTTAACCAATAAGCAATACTTAATCCCTCCAATCGCAGACTTTTGGTCAACTCAAACTGACCATTGAGGAGCTGGATCTGTCCTACAATCTCATCCGAAGGATACCGGAGAAGGCATTCGATGGCCTCAAGGATTCGCTGAACGAACTGCGACTGGCGAACAATTTGTTGGGCGACAATCTGAATCCCATCTTCTCCACCGCCGAACTGCATGTGCTGAAGAACCTGCGTCTCCTGGATTTGTCCGGAAACAAGATCAAGCTGATCGAGGAGGGTCTTCTCAAGGGGTGTATGGATCTGAAGGAGTTCTACATCGATCGCAATAGCCTGACATCGGTGCC

 

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