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CRIMIC CR02173

Gene m-cup

CG11896

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:17144475..17149412	89F1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	CAACAGTCGTCTGTTACAGCAGCTGT	ATCAACAGTTTCGTCGTATCCAGCCG

Images

Construct Design Sequences

sgRNA sequence	GTCTTTGCGTCCCTAGACGAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCATGGACAACTTCCCCGAA	CGAAGGTCCTTTGCACGAA	GCAAAGACAGATGGTGGCAT	TATCGCCTCCGGTACTCCAT

 

Amplicon 5'

Amplicon 3'

CCATGGACAACTTCCCCGAAACGACGGCCGCCCATAGTGGCGACACATAGTGAAAGCTATTGTCCTCCGGCACCTCGAAGTGGCCCCTCTGTTCGATGTTCGCCTCGCAAATGGTGATCAGATACTCAGCGATGTCCACGGCACCTCGTTTACAGGCGATAAAGAGGGGCGCGCAGCCATTTCGCTGCTTTCGCACCAACTCGCGACGAGAATCGCGGGGTAGCCTGAAAGGAGATAACGAATCGATGTAAATAAACTTTAAAATAATTATTATTCTTCGAATATTCTTCGGAATTTCTTTCTGTGCAAGGTAGGGTAGAGAATCGGAAGCTCTTGATCAGCACGTGCAGGGGCAATGATTTTGTGACCATTTGTTGATAAGATCGAGCTTTCAGCCGAGAATTCACACATTTGAATTTAATCAGGTATTTGTGATTGCAGAAAATATTTGGATGTTTACAAAAGCCTTAAACCAATGAAGCTATATATATATATATATATATATATATCAAATCAACAGGACAGTCATTTATCTATTATAAAATCTGCTTAATGAGGTAATCAAATTTCGAGAACGATTATAACATGTATAATACGCAGTATTCGTAAGTCTATAACGAAAGATTAAGTTATGAGGTGCGTTGTTTTGAAAAAGGTGGCTTCCCTATAATTTTAATACTTTATTTATCAGTAAAGTCAGGCGATTCGATTAGTTCCATTTGTCCGTCTGTTTAATATTGGTGTTTAGAGGTAATCATAACAACACTGACCAAAATCTTTTGTAGAAAAAGTTTAAGAAATGAATTGTTTCTACTTATGGAATAGTTCGTGCAAAGGACCTTCG

GCAAAGACAGATGGTGGCATTGTAAATTATTTATTATAAGTTTCAGCCAAATGTTGTTGCTTTCAAATTAGCAGGAATTAAAGTACTGTATCCCTAAGATGGAAGTCAAATATATGCTGGTTTCACTGCAGACACTATTATTATTGATTTAGGAAAACCTAGTTATTGGTCATTGAAATGAAGAATGGTATGGAATGGAGCCACGGAACCATTTAAAGGACTTGTTCATTACGAAACGCTCTACACGTGGAAGCCAATTACAGGGACATACTTTTCTAGTTCGTGGCGCAGGTGGCGCGACAGCAGCGAGTCCTCCTGGCTGCGTTTACATTCCTCGATCAGGTCGTGACTGAGCTTTGTTAGCTCCACCTTGGGACTGCTCTGCTTGCGTTCGTTGGCCATCGATGGCCGTGCGACTGACTGCTCCTGCTCTTGTCGGGTATTGAGCTCCTGATCCTGATCCTTGGCTTCCAGAACCAGATGCAGCTGCTCGACCTGTCGACGACGGCTGCCTTCAAGTGAGCGCATTGCCAGCGCACCGTCTCATTGCCAATTGATGGGTGGCTGGCTCTATATCCTTTGCAGTGGGTGTAGATGGGTGGGTGGTGGATGGTGTATGGCCTCCTCCCGGATGTCCTTGGGCCACTGCCCCCTTGCGAAAACGGTGCGGCAGTGACCTACTGAACCCACCAGCCTGGGCTACTTAATTGGGTTGCTTATCGCTGCTCCCGTTTTTCAGCCCAAGGCCGTCCGAACACTTTCTTTTTCTCTCTCTGTATCTCCGCTTGGTTGCCTTTCCTTCTTCTCTTCGAATCAATTTCTATGCAGCGTAAAACTTGGGTGCCTAATCTATGGAGTACCGGAGGCGATA

 

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