• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR02174

Gene TFAM

CG4217

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:20575245..20577010	92F2	FBti0213481	91462

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGCAGCACTTGGCTGAAGTAACTCA	GAGTGAGAGCTGTTTAGAAGGAACGC

Images

Construct Design Sequences

sgRNA sequence	TGCTAAATATTAGGACCACGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAGCCTTTTAAGGGGGCCAT	TTTTTCTCGTGGGCGCGTTA	CACTAAGAAACGAGTTCCATGCC	AAGCAGGTTCGCCATTGAGA

 

Amplicon 5'

Amplicon 3'

GAGCCTTTTAAGGGGGCCATTAAAAACTACATAATCCGTCCCTGATTAAACACTCGAACGGTTATCAATTTTAGTTGCCTGCCTTGTGCGCCGAATTCGCCGACCTCAGCACAAATCACAAATTCGGATCGGAGAGATATAACTACATATGTACGTTTGCAGAGAGGGAGAGCGGAAATATCATTCCAGTCGCCGGCATTCCAGTTCCATGCTGACCAGCCGCTTCGCTTCGAGTCGAAACGGTGCGTTCACTTCAAAACTGAACCGAGAAGCGAGCGGTTGTGTCTGTCCCACGGCCTAGCCCTCACAGTACTGCCAGTCCAGGCTTGACTGTACCTTTAGAACCTTTGTAATCTGCGCATTTGAGGTGACAAATCCATCGCATATTTGTTTACCGTGATCTACGCAGAGAGATTAAAACTGTGTCGATTACCGTGTTAAATCATCTAAAAACCAGTGTGATATTAACCCCGTAAGACTCAGACATGTGCTGCCAAAGACTACACTTTTTGGCCAGCTCGCTTTGAAGCCCCAAGTAGCCGATGCCACGTGTTTGCTCCACATGCAACGTGTTGGCCGAAGCGATCTTACAAGGGCTAGATCTATTCAACTTATAACTTGGCCACGATGGTTACATGCACTCGCTCTCTAGTCAGTTTAAAAATGTATTTTCATATATGTACAATATTTAGTCGGTTGCTGTATTTTTATTTCATGCTGAAACTACAGACTTAAGTTGTCCAGAAAAACTACTAGAAGTACCCTTCGTAAAACTAACAGCAGGAACACGGGGTTTAAGTTAAACTTATGCTGGCTGGCTAGCATTTAATCTTTAATGCCCTAACCTTAACAATTGGCGATCGAAAAAGAAAAACGAAAAACAGAATGATGCATGATTTGTGAATTATGTGATGGAAGAGGGCGGGCCGAGCAGCTACAACTATATATCTTTGGAGGCCAGCGTCTTGCGGGGCTTTGGCTCAGGTGGATCGATAAGATTTCCGTGACGCACCACGTCGATGTGGCCCAGGCGGATCATCTTCTCCTCCCAAACGGAAATCGCTTTCCTAAAACGCACAAAAAAGGTTGTGTTTTTTAGAAACTGTGTTCACTGGTTCAAGACATTATTTGTATTGAGTAAATAACGCGCCCACGAGAAAAA

CACTAAGAAACGAGTTCCATGCCAAAATATTGATTAATATAGAAGCGTTAATAATAAGCATTAACACAAGCACATAAAGATTTCTACTGTAGGCAAGTTGGACTTTAAAAAGTTTATAAAAGATCTTAGAGAAGTTAATGGTTCAAACTTGAAGTAGAGGGCGATTTATCATGAATTTGGCAGAATATAATAAATTGTATCTAAAGTTTTTACAAAAATCTGCACCTAGTTGTTGTTGCTAAATTTAAAAATTTTTAAATCAAAGTTTAAATAATAATCAATATAATTTTACTGTAACCTAGTCAGTGGAATGCAATGAAACTATGATTCAAACTAGAAAATCTGCTAACGATTAGTAAAATTCGCCAACTTGAATGCTGAGATGAACACGCACCTGTAGAGCTCCATCTCCTTGCGCGACTCCTGCATGTAGACCTCCTTCTCGGAGTCGGAAAGGCGAGTCCACTTGGCGGTGGTCTTTTGGTGCCACTCGCGGTAGGTTTGCTTGTCGCCCTGCGGAGTGTTGATACGTTCGCTGGCGATGAATCGCAGGAAGGCCGAAGCGGGCTTTTTGGGTCGCCCCAGCTCCTTGACCCGCTTGCGCAGCTGGCGGCGCTCCTTGGCGTCAACGAGGTCCTGCTTGAGCTGCTTGATCTCGGCCCGCTGCTCCTCCGTGAGTGTGGCATCGTACTTTGTTCGCTCCTCCACGTAGATTTGTTGGTCCCGCTTGAACTCGGCCTGCAGGCGCTCCTTCAGCTGCGCATCGGCATCGGACCAGTTCTTAGACAGCTGGCGCACCACCTCGACGGTGGTAATCTGGGGATTGGCAGCCTTCAGCTTGGGCCGCTGCTCCCGCATGAAGCGAAAGTAGGGAGTCAGCGGTTTCTTTGGTCGCGGCGGCAGGCCCAACTGCTCCTCCAGGGTCTTCGACGGCACCGCCGGAGTGTTGCTGATGCTGGCGGCTGCTAGGGGCCTAGGGGGTGTGAGGGGTCGGTGGTCGGTGTCATTGGCACGAGATGGATCACTGGGTGGCCAATCTTTGGCTACAACTTGCCTGACTTTGTTGATCAGCGAGCCGATGAGGCCGCCGCGCGAGGACATCAGTGTTGTGGTGTAGATCATGATCTCAATGGCGAACCTGCTT

 

---

Search Again