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CRIMIC CR02177

Gene CG42788

CG42788

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:15095957..15131926	88E1-88E2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGACACGAACATCATCGCCTCAA	CCTGAATTCAAACATGCCATTTAAGC

Images

Construct Design Sequences

sgRNA sequence	TGGCACATGGATGCGGGACTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCCCAATTGAATAGCTCCTGGT	CTGGGTGACTGCCACTGG	CGCATCCATGTGCCATGTG	CAAGGAGGGGAGGCTCTACT

 

Amplicon 5'

Amplicon 3'

CCCCAATTGAATAGCTCCTGGTTGTTTTCCATACATAGCCGTTCTATTTATCTTCTGTATTTATAAATCATGCAAGAGCTCGCCCATTTGTACTTGACCCGCCTCCCACGTTGACCCTGAACCTGCGGTCAACTGTTGAACAGTTGCAGCCCCATTCCACAACCACCGGGAAAGCCCTTCGTGGCCACGCCCATCACAACAGCAGATGTCTGTTTAGCCCTGTGGGTGAGCTAGAAATGCATTCCAATTGACATTAATCAGAGCTAATTGGGTTATTTGCTCGACTTTGCAATAAACAGCAACCCATAATCGGGCCGCAGAGCTAAAACTAAACCTAGCCTCAGTCCACAGCCCGTTTAAAAGCCACTACAGTGGAGCTTCGAACAAGTGAACTATATAAATTTAAATTTGAAATTCTCTTCAATTCAGTTATGGTTTAATACTTCAAGTCTATAAAAGTGTTTACTAAAAACATAAACTACTTCATTTATAGGCTACTCATCGAAGCTCGACTGTGGCATCCCGGGCAGGTCCCGAACCCTCGGGCCAGAGCACCAGCCAAATCCAATTGCGACCCACATCAGCCACCACCTTGGTGGCTTCCGTTGCTGTCTTTCTTCATTTCCTGACTGCACACAATTGTGATAATTAGTTTTGCACTTTTGCACACTCTCGACGAGCGAAGGATTGGTGGTGGCGGCGGCGGGGGAATGGGTTTGGCTATGGGTATGGGCACCCAACATGGAATCCAAACGGCTATGTTCACTCATTCAGTCTATCGCCCCAGTGGCAGTCACCCAG

CGCATCCATGTGCCATGTGCCGCCTCCGCTGCTCCCTGCCCCGTTTGCTTTCCACTCGGTTATGGCATTTTGTGCGCTTTCAGTTTGATGTGCAACAAAGTGTAAAATTATTTAATAGAATTTCGTGCATAATCAATTTATGTGCAGCAGAGAGCCCAGCATTCAGTCGCCCATTCAGCGTCCAAGCCATCTAGCCACATAGCCAAGCAAGGACCTTCCGTCAGTCAGTCAGTCAGTCAGTTAGTCAGTCAGTCAGGCAGTCTGTCATCCTGTGAGTTTTCCAGTTCGCCAGTGGGCCAGCATTGAAATGCTGAAACTTTGCCCATGCTAATGGCTAAATAAATGTCCGACTTTCTGCTTTGTTAAATATTCTTTTTTGTGCGTGGCGAAGGCAAAAAAAAAAAAAGTGGCGAGGTTATTAGAGTGGTTTTTGAAAAAACCACGAACGAAAGTTTGCCACAAATAATAAATGTTGCAGGGCAAAAAGAATTATTTTTAAGTTCACTTGTTAAAATGTAGACATTAAGCCTTTAAATCTTAGTTCCGTTTGCTAGATTTCTATTTATATATTTATTTCTATTGCTAGCTATATTTCTTGAACGCTTAATTGGCACACAGACCATACATATATCATTTAAAGTATCTCCAGAGTTCTGAAACGATTCCTAATTGCCAATGGAAATAAATTTCATTCTGTGCAGCAGTAATTTAAAAGTGCACAAAACTTTCTTAATTGGGCAAACTGGTAGCTAGTTTTATTCATATTGCACAATTTAAAACCATTAAAGAGTTTGCTGGCGTAATTCGAAAATATTTTTCAATGACATACTACGGTATCATATTACAGTATTATGCCATTTAAGTCATATTTTTCAATTTCCTGCGAATCTTATTCACAACTCACTTGCAATAGTAGAGCCTCCCCTCCTTG

 

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