CR02178

CRIMIC CR02178

Gene Dip-B

CG9285

Information

Donor Location Cytology FBti Stock number
pM37-p23R:13782288..1378567687F13Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
short band OK GCAAACCACAAACAGCTGTCACTTGG TTGCAGTTGACTGACCACCTGCATGA

Images

Construct Design Sequences

sgRNA sequence AAGGGGAACACACTGAAACATGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CAGATCCCGCTCCCAAGTTT TTTACCCTCATTCCTGGCGC CATGGCCATGGTTGCTCCTA ACTCCTTCTGCAACACCTGG
 
Amplicon 5' Amplicon 3'
CAGATCCCGCTCCCAAGTTTCCAGTTTCCAGCTGCAGCGCTTTGTTCCCATCAGGGAGGTTCAGTGACGCCCCACTTGCCGATCAACCAAGTCGCGTGAGCTGCCTGCGAAAAAGTACTCATCATGGGTATCGAAAAGTGAGTATTTATTACCAACAGTTATTAGAGGGTGTGTTTTAAGTTAGCGCGGATCAGTTATCTAGCCACCGCTTAGCGACCGATTGGAAAAGCTCTCAAAGATTCGCCATCTTTTACAGTGAGGGTTAGTCGCTTAGCATCCATCAATTGCTATCTTATTTACCTTTCCCATAATTCCAAAACGAAATTAGAATGTTTTTAGTACTGCATATCTCAGCGTTTATCAGTTTTAATTAGTTGCCGGCAAGGAATAGATGTCATGAAAATTCCAAACTTTATCTTTTCCCAAAATAAAAACAATGTTTGGCGAAATACTGTATAAAAGTTAAAGGGGGGAGGTCACTTAATTTTATCATTATTACTTAGATAACGAGTTCTTGCTACTTCTGGAAACAGATATATATAGATCAACTTTTATTGTTTGATATGGGGCACTTCAAAAGATAACACAATTAAATGGTCAGCGACATAGGATTATTCTCCAAAATATTATAGACCCAGTTACCTAAGGATTGGTATTTAGTAGAAACACTTGAATAAGATTACAAATATTCTAAATAAGTACTTTAACACCATTTGTGCTAATACTTGGTACACTTGTTTCTAAATTGGAAATCGGTTTGTGATTATGGTCATTTCGAAAGCTTATCGCGATTCTTTATAGCACTTCGAGTTAAGATAAGCCCAGCGCCAGGAATGAGGGTAAA CATGGCCATGGTTGCTCCTAATACAGTGGGGACATTTTCTTTTGAAATAATAAATTGAAATACAAAGGCAACAGGCTGTTATGCAATATGAACGACTGCATCAGTTAATAAAGCAAATGTGACGTGATAAAATTCAATTCAATTAAACGCTATTGCACATTTAGTTGACACCCAGAAATGTTTCTTAATGAATACCAATAACGATTCCGTTGCGCACTTTAACTCTACTCTACGTGTGCCCCTCCAGAATTCTACCATGCACCCTCCGGCTGAACAAGCTGATGAGTCTGGCCGGCAACGACTGCCTGTGCATCATCGATCGCAATGCGGTTCCCAATGAACTGAAGGCCACCTTCGAGGAGCACCGCAAATTCGACAAGTCCTTCGACAGCAGCATATCCTGCTTTAAGGTGCCCAACGTGGATCAACCTGTGGTCTATGCCCCCGTCTCCGAGCTCACCGACTACGACGATGTGAGGAGCTACCAGGAGGCGGCCAAGCGGTCCATGGAGAAGGTTCTCAAGGTGGGTTTTTTTTTTCTATACATATTGTGTGAATTATGAAAGAAAATGTATATGTATTCTTTGCCAGGCTGGATTCCACACGCCGTTGCTGTTTGTGCCCAAGGTTAAGCGCTTCCCCGAGGTCGAACTCTGCACCGTTCTGGGAGCTCTGGAGCAGCTTTATGTGGTAATATGGATTATGTTTACTTTTATTTCAATCAATGTGCTAAAACACGTTTTTTAGCCCATTCAATTGCGCGAGGCAGGAACTTTGAAGGACCCCCGTGTGACCACGCTCAGTGTCCAGATCGATGATCCAAGGGCCGAAGCTATTTTCCAGGAAGCTCTCATTCTGGAGGCCGGTCGCTTTGTGGCCCGCGACATCGGAGTTGGGGACCCGGAGCGCATGACGCCCATCCAGGTGGAGAAGTACATCAAGCCGTTGTTCGACAAGCTCAACGTGAATGTGATCAGTGACACCCAGGTGTTGCAGAAGGAGT
 
Search Again