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CRIMIC CR02180

Gene CG5646

CG5646

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:27559379..27561319	98A6	FBti0213482	91463

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	GATCAGCTAGCCAAGGCAGCAACAAT	CTAAGGCTCCTAATCTCCACCAGACT

Images

Construct Design Sequences

sgRNA sequence	AGGCAGTTCCCCTCAACAAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCAGCAGGTTCACACTGGAT	GTTGAGGGGAACTGCCTCAG	CTGCTGCATTGCGTCATGC	TGCTTTCGAATCCAGGCAGA

 

Amplicon 5'

Amplicon 3'

CCAGCAGGTTCACACTGGATCCGTCCGACTGACTGCGTCGCCGGCGCACAAAGTCCCGCCTGTCCATGTAGTCAACGCCCTGGCGATAGGCCAGCATATAGATTCCATAGGGCAGGACGTCGCTGTAACCAAACGACCATTGAAAGATTCATGTATTTTTGGACTACCACCTGGACTAACTTACCGGCACATCATGGGCAAAAGGCCGCGATAGAGCCCCGAGATGCCATCAGTCTTGAGTATCCTTTTGAACGTGCCAAAGGCGGTCCTTCTCTGGCCATACAGATAGTCACTGTAATCTACGGAATTTCACAAATTAATCCTCCATTTTGGGATTAAAAAATCAAGTAGAACCCACAAGTGGCTGTCTGCAGGCGAACCTTGATCAGCTCCATGGGACAGGCGATGAAGGACTGCACGAATCCCGCCACAGAGCCCGCCAGGAACATGTTGTGGTACTCCAGCTGCTCCCGCTGGTAGTCACTGTGGCACACCTTTCTCAGCTGCCGCAGATGGTTTCCATAGATGCCGAAAAGCAGCGAGTTGATCGCTCCCGTCGAGATGAACGGGAAGAACATGCCCCTGTAGAAGCCGTTGACCTTTGAGGCGATGCTGATCGATTAAACCCATCACGACCCACCTAGGCGCTCTAAGGGATTGCGTCCAGTCGGAGTCGGGATTAAGAATAGGACAAAGCCATTTTGGCTGGTTTAAATATTTGAAAAGTTATGAATAAAAAAGGAGATTGGAAGATCGACATGTACATCGTTATCCAAATATTTCACATAAAATTTGTATCCTTGAAAAAAAGTTTCCAACTAGACGCAATCCTTCTTTCCAAACTTACCCCATTATTTCTGCTGTAGATCTGTTGAATGGCTGTCACAACGGATGAATTTGAGGCCTGTTGCCAAACTTTGATCGTGTCGAGTGGGTGGGCCACCAGCACCCCGCAGGCACCTGAATATCAGAGTATAAAAATCAAGAATGCAGCATAAATTAATTAAAATTGTGGCCACAAAGTTAAACAACACATGTTGAACGGCCGCCGCCGCCGACAGCCGCTTGCCAAGAATCCGATGGATCAGGGGCGCCGCACGTGAACTTGACCATGGCGCCCGTTTTCCGCCGGGACTGAGGCAGTTCCCCTCAAC

CTGCTGCATTGCGTCATGCATGGCCCAGAGTTCTGACCAAATTGGGATGAATACCAGCTGGAGCTCTGCCGCTCCGTCACTCACCACCAAAGCATCCGGCCACAAAGTCGCAGTAGTTCTCCCACTTCATGGATGCGGATTTTCAGCGAACGCTTGGAGTTTTCGGAAAATTGATTTTTCTCGCGATTTTCCAACTGCACCTCACGACGGCAGCGAACCAACTGCAGTGCACAGCTTTTGTGGCTCGGCAGCAAAGCTCTGCGATTTCGCCAGCTTTCCCAGCGAACAGAAGCTGATTGCTAAAAAATAGGCAAACCGAATGCTCACTTTTCAAAAAAAGATACTTTCTAACGAAAAAGTAACTCTGCTAAGCATAATGATATCTTGTGGGTATACAGCTCAAAACTATTCAGTTCTAAACTTAAAATTTTCAAACCAAACTTTCTAGAATTTTTCCTACGCATAGGAAAAAGAAGTGTAGAAAGTTTCTTAGCTTTTCACTTCAAGTTCAAAGGAATATAAATCCTTTAATTTTAAAAATACTATTTTGAATCCTATCTAAAATCAAACACGTTGTGTTAATTATTTTGTATTTATTAATTTTTTATTGTTGATTGATCTCTTTGCTTCTTTTCTCTCCGTTTGCTCATCATTCTCATTCATTCAGTTTTTGTGTGTTTTTTTTTTGTTTCATCTTTTACTTGGTTTTTAATCATTTTTGGTTAATTCTGCTTGCAGCTAAATATTTGTTTTTGCCTGCGCCATGCAATTTTAATTATTTTTTTTTCTGCTGCTTCTCGCCATTAGTTAGTTAATTTACTTTATTTTCGCATACTTTCTGCCTGGATTCGAAAGCA

 

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