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CRIMIC CR02183

Gene CG2246

CG2246

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:30592971..30601489	99F10-100A1	FBti0213484	91465

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTCCACCTCGGACATTGTCATCATCA	GGACAAAGTTGTGGGATCTTGGAC

Images

Construct Design Sequences

sgRNA sequence	AAAAAGTGTCGAGGATTTCGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGTCCTCCAGCGTTACGTTG	CGGGCATACGGAGCAGTTC	CGTGGCAGAGCGGAACAATA	AAGCTGCTGGCCAAGATGAT

 

Amplicon 5'

Amplicon 3'

AGTCCTCCAGCGTTACGTTGCGGAACAGATAGGACATGGACTCCTTGTTGTGAATGCGGCGAATGGCCTCAGCGATCAGAATGGAGATGTCGATCGTCTTGATCTTATGGCACTGCAGCTTTTGGATCTCGTGTGGAATGGTATTGGTTACAACGATCTGTCGAGGGAGATAGTAAACAAAATTATGAGTCATATTCAGGGCAATTAACTCCGGGCGGCACATACCTCATCGATGGGCGACTCGTCCAGCTGACGCGGTGCATCTGAGCTAAGCAGACCATGCGTGGCCAGCACGTATATCTTGCAGGCACCGTTCTCCTTCAGCATCTCAGCGGCGGCCACGAAGGCCTGCACATCGTCGATCAGGTCATCCTGATTAAAGCAAATACATTCAAAGTTAATTTCGGAGAAAGCCTTGGGACGCAGATCCAGGGTACTCACCACCATGATGGCGATGCGTCCACTGACATCTCCCACAATGGTCAGCGGTGGCTTGACCTTGACAATGTGCTCCGGAACACCCACCGAAACCGAGGTGGTTCGCTGGCGGGCGCTGCTGGTTGGGGCGTGTTGTGGAGTAGAGTTTCTAGTGGTTAGTGTGGGGCAGTATCACGTGGTTTGCAGTTCGTGTGGTGCAGTGTGGTGCATTGTGGTACATTGTGAGTTGGTTTTTGTCGAGGTGGTGGTGCATTGCATTGTTTGGTCGAATCGTAATCGAAACCAAAAAAGAAAAGAATCGTGAGGGTGGGTTGGGAGTTGGTGGAGTGGCATGGGTGGGGAAAGATACAAATGACAGTCGGTTAAAGAATACAATTAAACTGAAATTAAACAATAGTTTAAAATACGAACCTAGGCGTCGCTGGTAAGCACATTTCAACTGAATTTCCTAATAAATTGCTATACGCACTGCATAACACAAATAAAGAGAAATTTCGATTGTTTTTCTGTTTTCTGGATTTTAAATTTGGACAAGAGAACTGCTCCGTATGCCCG

CGTGGCAGAGCGGAACAATAAATTTAAACAAAAACAATTTCAAAACGCAAAAGAGGAAACAAAACAATTCAAAGCGTTTTAATAGGATCTAGCTTTTAGTTTAAAGTCTACTAATTAAGTGTCTGCCTTGGCCGTGAAGTACGAACAGAAGCCACTGACAGAAGCTCAAAATATATGGCATTGTGATGGTGCTTTTGTGAGTATGTGCGTTAAAAATTAAATTTATTCAAACAGAATGCAAAGCTACAATGCTGAAGAACTAAATTGCATTTAAAAAATCACAAAAAAAAAAGAGTGGATGATTGATATTCGAGTGCTTTACACCCGACGAACAAGAACGAGTGCAGAGTGAAATTATCTGGATTTGAGGTGAGTTAAGCTTCGGTCGCAGTATAAAGTTTCAGAAACTCGAAAACTAACAAGAAAAGCTAACAACAAACTATAATAAATCAGCTAACAAATCAAACAGGAACCTCACAACGAAATAACTTAAGCTAGGAAGGATCGAGGCTACGGGCAAGACTGAGGGAATGTAACTCACCTGGTCGGTGGAGGGGAGTACCGACCGTCGACCTCATCGGCATCCGTTTCCTTCTGTTCGCCATGGATGACGGCCAGGCCAAGACGCAGTCGCTCGGCGTAGGAGTTGGCCTTCTTGGCCACGCCCGGATTGCGAGCTACAATCACCGAATTTCGATAGTCGGGAATCTAGAAGCATTATCACATTTAGTTTCAATCTTCGCTTAGTTTGCACACTACCCACGCTCTCTTGAATATATTGGAGCAGAAAGGGCGACGCCCTCAGATTGTCCACGGGTATGTCAAAGAAGCCCTGGATTTCCTTCTGGTGCAGATCCATAGTGATGATGTGCGTCAGGCCAGAGGTGCACATCATCTTGGCCAGCAGCTT

 

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