CR02183

CRIMIC CR02183

Gene CG2246

CG2246

Information

Donor Location Cytology FBti Stock number
pM37-p23R:30592971..3060148999F10-100A1FBti0213484 91465

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK CTCCACCTCGGACATTGTCATCATCA GGACAAAGTTGTGGGATCTTGGAC

Images

Construct Design Sequences

sgRNA sequence AAAAAGTGTCGAGGATTTCGTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AGTCCTCCAGCGTTACGTTG CGGGCATACGGAGCAGTTC CGTGGCAGAGCGGAACAATA AAGCTGCTGGCCAAGATGAT
 
Amplicon 5' Amplicon 3'
AGTCCTCCAGCGTTACGTTGCGGAACAGATAGGACATGGACTCCTTGTTGTGAATGCGGCGAATGGCCTCAGCGATCAGAATGGAGATGTCGATCGTCTTGATCTTATGGCACTGCAGCTTTTGGATCTCGTGTGGAATGGTATTGGTTACAACGATCTGTCGAGGGAGATAGTAAACAAAATTATGAGTCATATTCAGGGCAATTAACTCCGGGCGGCACATACCTCATCGATGGGCGACTCGTCCAGCTGACGCGGTGCATCTGAGCTAAGCAGACCATGCGTGGCCAGCACGTATATCTTGCAGGCACCGTTCTCCTTCAGCATCTCAGCGGCGGCCACGAAGGCCTGCACATCGTCGATCAGGTCATCCTGATTAAAGCAAATACATTCAAAGTTAATTTCGGAGAAAGCCTTGGGACGCAGATCCAGGGTACTCACCACCATGATGGCGATGCGTCCACTGACATCTCCCACAATGGTCAGCGGTGGCTTGACCTTGACAATGTGCTCCGGAACACCCACCGAAACCGAGGTGGTTCGCTGGCGGGCGCTGCTGGTTGGGGCGTGTTGTGGAGTAGAGTTTCTAGTGGTTAGTGTGGGGCAGTATCACGTGGTTTGCAGTTCGTGTGGTGCAGTGTGGTGCATTGTGGTACATTGTGAGTTGGTTTTTGTCGAGGTGGTGGTGCATTGCATTGTTTGGTCGAATCGTAATCGAAACCAAAAAAGAAAAGAATCGTGAGGGTGGGTTGGGAGTTGGTGGAGTGGCATGGGTGGGGAAAGATACAAATGACAGTCGGTTAAAGAATACAATTAAACTGAAATTAAACAATAGTTTAAAATACGAACCTAGGCGTCGCTGGTAAGCACATTTCAACTGAATTTCCTAATAAATTGCTATACGCACTGCATAACACAAATAAAGAGAAATTTCGATTGTTTTTCTGTTTTCTGGATTTTAAATTTGGACAAGAGAACTGCTCCGTATGCCCG CGTGGCAGAGCGGAACAATAAATTTAAACAAAAACAATTTCAAAACGCAAAAGAGGAAACAAAACAATTCAAAGCGTTTTAATAGGATCTAGCTTTTAGTTTAAAGTCTACTAATTAAGTGTCTGCCTTGGCCGTGAAGTACGAACAGAAGCCACTGACAGAAGCTCAAAATATATGGCATTGTGATGGTGCTTTTGTGAGTATGTGCGTTAAAAATTAAATTTATTCAAACAGAATGCAAAGCTACAATGCTGAAGAACTAAATTGCATTTAAAAAATCACAAAAAAAAAAGAGTGGATGATTGATATTCGAGTGCTTTACACCCGACGAACAAGAACGAGTGCAGAGTGAAATTATCTGGATTTGAGGTGAGTTAAGCTTCGGTCGCAGTATAAAGTTTCAGAAACTCGAAAACTAACAAGAAAAGCTAACAACAAACTATAATAAATCAGCTAACAAATCAAACAGGAACCTCACAACGAAATAACTTAAGCTAGGAAGGATCGAGGCTACGGGCAAGACTGAGGGAATGTAACTCACCTGGTCGGTGGAGGGGAGTACCGACCGTCGACCTCATCGGCATCCGTTTCCTTCTGTTCGCCATGGATGACGGCCAGGCCAAGACGCAGTCGCTCGGCGTAGGAGTTGGCCTTCTTGGCCACGCCCGGATTGCGAGCTACAATCACCGAATTTCGATAGTCGGGAATCTAGAAGCATTATCACATTTAGTTTCAATCTTCGCTTAGTTTGCACACTACCCACGCTCTCTTGAATATATTGGAGCAGAAAGGGCGACGCCCTCAGATTGTCCACGGGTATGTCAAAGAAGCCCTGGATTTCCTTCTGGTGCAGATCCATAGTGATGATGTGCGTCAGGCCAGAGGTGCACATCATCTTGGCCAGCAGCTT
 

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