CR02184

CRIMIC CR02184

Gene disp

CG2019

Information

Donor Location Cytology FBti Stock number
pM37-p23R:5849697..585631183C5FBti0213485 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK GAAGGCTACTAGTTCTCCCTCTCCAA GCAGCCAGAGATAGGCATAGTTCATG

Images

Construct Design Sequences

sgRNA sequence TGTACATGAACGATTACTCTCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TGTTCTCCAGGGTGGGAAGA ACACCGATCTACGAAGGTCC CTTTTGTAAGTCGACGGCGC TATGGCCACAACACCACCTC
 
Amplicon 5' Amplicon 3'
TGTTCTCCAGGGTGGGAAGAGCACTCTGGGACTGAGTGGTCAAGGTACATCGATTGCTGAACCTCTCGGTCAGCACACAGTGCCAGATCTTAAGAAACAGAAATACGTCGTCTGCTCCAATGCCAATTATTACTACCACGGCCAGAAGGTTCATATAGGGGAAGAACTCAAACTCCAGCACGATGGCGTAAAAAAAGTACGCTAGTCCTAGCGAAAAGCATATGGCTACGCAGGACATCAAGGTGATGAATGCCGATCCCGTGTACAACCACACGCTAGCCATGACGAATGTCCCTCCCAGCGAAACTAGCCACACGTCCGTCAGCAGCAGCTCGTTAAAGAGTTCGTTTTCGAGGCCCAAGTCCATCGCAACCACTTCCACAAGCTCGTTGATCAGCTCCCTGAAAAATCATTCAGAATATTATTATCAAACAGAAAACATCAAAAATATTTTTAAATAAAAGAAAAATACAAAAAAAATTGCAAAATGGATCCAGTTCAACGGGAAAAGGAATTAATTAAAATGGAATTGCCAAGGACGAGGGTGCTTGTAACCACCAGCAGAGCGGATTATGTGGATCATACTGAAAGAGCCGTTTACAATCCCCCGATTAAGAAAACCCAAGAGGATCCCTCTCTAATAAGTGGTCACTACTGCGCAAATCTAAAGGTTGGTGGCATTGAATATAAAACAAAGACATGGCCCCAATCAATGGACTGGCGGGAGGACTACGCACAGTTCATCAAGCGCAACAAGTGGTGTAACGAAAGGATCTACAAGCTATTTTTTATGTATCCGCCTGTGGATTTCCGAATTCTGAAGGACTTTGTGAAGGACCTTCGTAGATCGGTGT CTTTTGTAAGTCGACGGCGCTATAGGAACAAAAAAGGTTACTCTCTCAAGGACGAGGACTATCGGACCACATATGGGTGCTATTACAATTTCCTGCAGGAAACGGAGCCGTTTAGGAGCTCCTTCTATCCAGAACGTAGGCTAAAGGACACTACTCTGGACAAAATCGCCAGCGATTTACGAAAAATTTTCACAAAGTACAACTTGACCACCTATTTTGATACCATATGCGTTCCGGCCTTGATGAAAGCTAAAAATAATGTAATGCCCTCCACACCCATCGACCGGTATCAATTTCGCAAATATTAGCTGTGCTCCATACTTACACGTCTTCCCATTCGTGGAACAGTGGCAGCAAGCGGTTGGATTGCGCCACGGGTATAAAAATCATGGCGTATTTCAGGTATACATTCGAGTCCTACAAAAAAAAAAAGTGCCAATATTATATGGTCGAATTTAATGCAATACGAGTAAAGCAACGGACAAATACAAATCTGTTTTAATAAAAGAGTTTTGGGGTCTAAGCTTATTTTAAACTCAATAACTTACATTAGACTTGATGAAACTAAAGTCGGTGAGAAAATTCAGGACGTTAAAGACGATATTTAGCCGCTTGCACTCTTCAGGAGCACGACAATGCGGTATTTCGTTGCAGTGGTTGTCCATCTTTAGGTCATGGAAGTACTCATAGCAGCCCAAGAGCAGCGTGTGCAGCGAAGTAACATCCTCCGTGGTAAGGTCGAAACAGGAACTTTTGTTGGCCAGCATGGCGGCGTAGTTGGGCAGTGACCATGGCCGGCAGCACTCGGTGGTAAGCATCTCCGGCTCGCAAAATGCCCGGTAGCTGGGGACTTCGGTAATCTGATCCTGCAACTGACACATGGCCAGCAGTCCGTTCAAATCAAATAGAGAATCGGTGGCGTTGGGACCGATCCGCTGGACCACAAAATGAGAGTACTCCTTCCGGGGCGATGAATCGCAAAAGAATCCCTCGATGGGTTGGCGACGATGCATGTCTGCCCAACCGTCGGTGGGCAAGGTGGCGGCTTGCTTTAGGAGCCGCCAAGTGCTCTTCTTGGTCTGCACCCGCTGCTGATGCTCTTCCTCGTCCACTGAGGTGGTGTTGTGGCCATA
 
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