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CRIMIC CR02187

Gene CG5854

CG5854

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:23933912..23935964	95C13	FBti0213486	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCACTCAAAGATGATCCATCGTGGT	GGTGCCATGTGCTTGTCGTTGATT

Images

Construct Design Sequences

sgRNA sequence	GATAAGCGGAAGTGTGGAGTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATACAGCCATCCGATGAGCG	GGCAGGATGACGATGAGGAG	AGCATCGCTGACTGCATCG	GGGTATCAGCGAAGAGGGAC

 

Amplicon 5'

Amplicon 3'

ATACAGCCATCCGATGAGCGACAGTGCTTGGACGGACGAACACCGTTGCGCTTGCGCCCGCTATAGACCTTGGTGAAGGCCCCAACGCCGGCGGGACTGCGCAGGTACAGGTGTCTCATAATAGAGGCGCAGCGAGTGTAGAACCAGTCGTCGTCGGTAGGAGCAGTCTCCTTGAACTTGCCAGTCTTCATATAGACGGCCTGTTCGGGCACAAAAATTTTTCCGGATTTTTTGAGAAAAGCAGCCATGTTTTTGGTCAGAACGTGCTGATCGATTTCCTTTACTGTGACTCCAGGCATGTTGCTCTCTACGTTCTTGAAAGCACGTTTTTAAGGCTCATGGGTCCGATATATGTATGTATGTATTTAAAAAAGTCTTACCTTTTGAGAAGAAAAAGGTTTGCTTCCGGTTAGTTTGCTGACTTTAGTCAAAATGGACGATTGGCAAGTGACGTTTGAAAGAGGGACCAGGTCATTGACCCCAAAGGATTACTTTCCGATAGTTTTGTCGTGCAGTATTGGTGAACTTGGCAATTCTTTTCGAAAACTTAACCTATAAACTTGGAAAGGAACGCAATGTAGCAAAACTACTGTTCTTGGTGACAGGGGGTTTAAGGTAGACTAACAAGGACAATTTTATGACACTGAAGCCCTATGGAGTAAGAATCAAAGAACTGCTGTATTTTGGTTTGTATAAATGAATAAAACGTTCTACGCTAATTGAAGAGCATTCGAAGAGGTTTGAATACAGCGCCATAGGGTGACCAGCTTGTGGAGCATTGAAGGTATTTCTTGTTTTAAGAATGATCACGGGATGGTCACACTAGAAATACAGCCAAACAAAACAACAAAAAGCATTTCGAGCGCTAACAAATATATATCTTTCGACTTGACTCATTCGCATTCCGGTTGACCGTGTCGCGCCTGCAGCATGTCTGAAAAGCCGACTGTTCTGATTTTGGGTGGTAAGTGGTCCCGCTGCATCCTCCTCATCGTCATCCTGCC

AGCATCGCTGACTGCATCGCTGCTCTTCCTCTTCCCGCAGGCTGCGGCTTCATTGGACGCAACTTGGCCACTTATCTGCTGGACAATGAACTGGCGCAGGAAATACGACTGGCCGATAAGACCCCTCCGCAGATGGCATGGCTAAACGAGGAGCAGACGCGGGTCTTCGAGAGCGATCGGGTGGAGTTCTGCAGCGCGAACCTAATAAACGCCGGTAGGTAATCACTACATTAGGGTGGCTCGTTCCACCAGGATGCGGATCACAACTCCTATGGAGATCCAAAATCCGCAATTAACATTCTTGTGAAAGGAAAGCGCTATCGGATATAGAATTTTCAAGTACCACCTTGGTATCGCTGCGCGTAACGAGCCACCCTAATGCATTTGTTGCACCTAAACTGGTTATAGAACGCTTCCCTTTGTTTTCATACAGCCTCATGCAAGGCGGCTTTTGCGCCACACCCCACCACTGGACGGGCCTGGGACATTGTCATCAATTGTGCGGCCGAGACGCGTGCCAACCAGGATGATGCCGTATACAAGGAGGGTATCCTGAAGCTCAGTTTGAACTGCGCCAATGAGGCGGCTAACCAGCGGGTCAAACGATACGTGGAACTCAGCTCTGGTTGTGTGAACAGCAGCGAAAAGACGCCGTTAAAAGAGGACTGCAAGACGGATCCCTGGACGGGCGTGGCCAAGCAGAAGCTCAAGGTGGAGAAGGAACTGGCCAACATCGACGATCTCAGTTACACGGTCGTCCGTTTGCCCGTGGTCTATGGCATTGGCGATAAGCGCTATTTGAGTGAGTGTCCCTCTTCGCTGATACCC

 

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