CR02189

CRIMIC CR02189

Gene B52

CG10851

Information

Donor Location Cytology FBti Stock number
pM37-p23R:13661311..1366689187F7FBti0214969 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK ATCCTTCACTGAACGGTACGTGCT CCCACGTCTTTTCAATCAGTGTGG

Images

Construct Design Sequences

sgRNA sequence ATAAGCTGATTTCTTCTGCGTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GAAACATACACGGCGCGTAC TCAGCTTATACGATTGCACTACC CCAAAAGACACTGACTTGCTGT AGACGACGAATGGATGAGGC
 
Amplicon 5' Amplicon 3'
GAAACATACACGGCGCGTACATATGTGCGTTTTTTTTTTCTTCCATTCCTAAAGGACAGTCGAGCAAAATAGAAGCTGCAAACTGCAACGGTTCCCTTGCTGATATATATATATATACTTCACTATTTTATAGCACCTGCTCCAGATACGTAAGGAACCGTTATCATGGTGGGATCTCGAGTGTATGTGGGCGGTCTGCCCTACGGAGTGCGCGAGCGCGATTTGGAGCGCTTTTTCAAAGGCTACGGCCGCACACGCGACATCCTCATCAAAAATGGCTACGGCTTTGTGGTGAGTACAAAATATCATATTTAACTGGAATATGTAAAAAAAAAAAATCGGTTAAATTTGATTTGAAGACATTTCTGCATTTCTCTGTTTGTCTATAGGTTTCCTAACATTTCAAGCCGACCCTTGAATACTTTTTTTTTCTTTTTGAACCTATATTTGTTCCATCATTTGTCTGTCAAATACAGTGACGCTTAAAAACTGTTCTTAAATTCACCCAGCGTATTTCCATAAGAGTATGCACAGGCAGGGTAGCTTCAATACTAATTCCATAATCAATACTAATCACTAATTGAATTTTCAGGAATTCGAAGACTATCGTGATGCCGACGATGCCGTCTATGAACTGAATGGCAAAGAGCTGCTTGGCGAACGGTGAGTTGTTAGATATAAGCCAAAGTACGAAATGACTCACGATTGTATGTTCTATATTTTTGCAGTGTGGTTGTTGAACCCGCCAGGGGTACCGCTCGTGGCAGCAACCGCGACCGCTACGACGATCGATATGGTGGTCGGCGGGGGGGCGGGGGCGGTCGTTACAACGAAAAGTAAGTAGTACTGCCAGCGGACCTTAAAACCGGACCAAAGTAGCGACGATATCGTCCTCCAACTTTGTTTGCGTTGAAGTTTGCCCTAGAATTAGCACTGCCAATTTGTTTCGTTTGATATCTCCATCCACACAGATGCAACCAGCTTAGATCGTGCAAAATCTATATACCTATAATATTAGCTACTTGCGATATATATAAAAAACATCAGTTTATTATATATTGACTTCTATAGTTTTATACACTTTTGGTAGTGCAATCGTATAAGCTGA CCAAAAGACACTGACTTGCTGTTAATATTATCATTTCCAAGACGCTCCGTTTTCCTATGCATACTATTGTTGCTTTTATTTGCACGTTCCACCCATCCGCATAGTATGTAAAATGTTTGATTTGTTGCGCATAACCCAAATGTAAATATGCATATGCTGGAATATATTCACCATTACCGTTAATTTTTGTGATGGAGCATCAGGAGCAGCAGCGCAGTGTCAATACTAATTAGCGAATCCATTCAATTATTCACCAGAAACAAAAATTCCAGATCATCCTCTCGTTATGGCCCACCGTTGCGCACTGAGTACCGACTGATTGTGGAGAATTTGTCTAGCCGCGTTAGCTGGCAGGTAAGTGAGCATGGATCGATGTATCGCGCCTTGGGCGTGGTATATACTGTAGCTTAGATTCGAGGAGTGTAGTACTAGAATTAAAACGGCTAACCGACTGAAATGGATCACTATTACAAACACCCATTAATCAACTTACCCATCCCAGAACCAACCTTTTCGAGCCCCTTTTTGATTTCGAATTACTTTCCGGTGTCTTCGTCCTGTTTTGTGTTTTAACTCCTCCTCCACTCTGACTAGAGCCAATTCTATAATCTGTAATCTAGCCCTAAGCTGTACAGTTGACCTAGACTTAAGTGAAACACGAAAACAAAAGATATTGCTAAAACCCTGGTACTGATCGGTGTAATCTACCCACCATTACTTTCTCTTGCACACACCAACCGAAACCGATAATAAAAAGAGTTTGATGTGCTTTGACTGAGAGATTCCCTCCTCGGCTTGGGGCCGAATCTAAAACTGAAAGAGAATCAAATCTAATTGAATCGCTGTGAGAAAGACAAGCTAGTCTAGTGACCGACCGACCGGTTCAGAATGTTACCCAAAAACCAAGATGCGGTGGAAGACAGAGTGAGACAGTACCCAGAGCCGCCCCAGATTGGTTTCGATATGCGAACTACGATCTGCAACCTACGACCGACCTCCACGAAGCGCCATCGCCGCCGCCTCATCCATTCGTCGTCT
 
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