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CRIMIC CR02194

Gene CycG

CG11525

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:31596642..31612664	100C7	FBti0213487	91467

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCAAGCAGCTGGACTTGAAACCC	ACTGCTGTCCCATTCCAGATGTTC

Images

Construct Design Sequences

sgRNA sequence	CAGCTGGCTAAGATATACATAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGATGGTCCGCAGCATTCTT	AGCTGTTGTGCGTCCTTGAA	GGTATTCGAGTATATGCCTCACA	TGGTTGTACCGCTGGTGATC

 

Amplicon 5'

Amplicon 3'

TGATGGTCCGCAGCATTCTTTTTAATTTTTTTTTTATATTTTTTTTTGCTTTTGTTTTTTTTTCACGTATTTTTTTGTCTGCCGCAAATAAATCGAAAAAATGTGGGGGCGCTACTCTCGGGTAACTTTTCAAAAGGGGTGGGCGTGGCTGCGTCACCTTGGCGCCCACATTTCCGTATCTCAATAGTTTCTTAAATGTTAGAACCAGCGCGCTACCAAATACACATTTGTGTGTATGCCAAAAGAATTATGATGCACTTTAGCAAAATATACACACTCTACCTCAACCAGCACTACTACACTCGCACGCGGAACGCTCTCACGGACACACATCCTCTGCGGAGATTTCCACCCTCCTGCTGCTGCTATGGGATATGCCTAGTGCCAGGAAAGCAGGAGGTGTGAGGTGTCAGGTGTGTCCCGCGATTACGTCTGGTTGATGGTCCTTTTGTCCGCTGCTCCAGTTTTGCCCAGCTGTTGACCCTGCTGCTCTACTGCTGCTGGCTGTCGCTGGATACTAACATTGTTCGAAAATTGGAATTATGGGTGAAGTGGGCCAGTCCTCCTCCTCCTCGGAGCTAATACTCTCGGTTCTGGAATAAGAAACAAACAAATTTGCAATGTTAACAAAAAAAAACGTATCATACCAGTCAACCAGTAAAAGTGATTTATCGACCATTTGTTGAGTAACTAAAAAAAACCTTCTATCTAATACCCCCGTTTTTTAAGGCTTCTACCTTTTCATAACAATCGGACATCAATTAACAAATTAAGTTCAAGTTGAAGGCCCCTTTTGCAATCAAGGCCCCGAAAATCAGCTGATAATTCGCAAGCCTTTGCTCAGGTGCAACTGGAACACAAAGTTCGGGGAAACAGTGCATTGAACTTTTCGAATGTTTATGACGAACTTTTCGAAACACCGACAAAACCCTAGAAAACATTATCAACGATAAGATAGCAATTAGGGGGAAAGCTAGAAAAGAAAGAAAACAACCGGGCCGAAATAGAAATCCAATGACATTGAACATCCACATCTAAGTTCAATGTGAGAATTCAAGGACGCACAACAGCT

GGTATTCGAGTATATGCCTCACAGATATATATATATATGGGTTTTGCATAAGCGAATACTCTTTCCGCGACCCTTGCTGCGGTCTACGTGCGTGTATTTTGTTGTTGCGTCTAGCGGGTATTGTTGCCGATTGTTGCCGAAAAAAAAGGCAAAAACAAGGGCAGCGAAAATAAAAAACAAATTGCCACACACACAAACACCACGCATACATATATGTATGTAGAATGGGCGGTTCGCAGAGGGGGCGGACAGACGAACGGACGGACGGGCGGTGGGCGAGCCAGCCAGTCTCATGACAATTGTGTTGTTGCCGCTCTGGCTGATAATGGAATCGCTAATATTCTAGAAACTTGTTTACGCCAACAAGTTCAGATCGTCATCGACACATTTGCTTACATTCGAGATTATATATGCGCGTGTTGGCTATGTCCAAAATCACTAAAAATAAAGTCCGGTGATCCCTGCCGCAAAGTTCTTTGGTCCAATTTACAATTATGTTTGGAAAACTGCCCCTCTTAAGATAAGATCGCAGACTCACCTAGAACGCAGGCCATCGTCGAGGGCGTTGGGGATGCCTTCCTCAATGGTGGGCAGGTCGGAGGAGAAGCGGTTGATCGGGCGCAAGACGCGCAGCGTGCGACTGGACAGCTTCCAGACAAGCCGCTGCTTGTAGGGCGCCTTGTTCTGCCCGTTGTAATGGGACAGAATACCCGAAACGATGCTGAAGCCGCAGGTGAAAACGCGATCAGGAATCTGTGGGGCACACGAAGCCAGGACATTAGAGAGGTTCAAGTGAAAGTTCCAGTTTCTCAACGAAACTCACCCTCATGTACTGCTGCAGGAAGAGAATGTACTCGAAGACGTGCTTCAGCTCCGGACTGCCGCGGGTGTACGACTCCTTGATGTGGAAGTCCAGGTGCAGGCAGATGAGCACCAGCGCCAGCGTCGAGGGCGTGATCACCGTGGTCTTCACGTCGCACATCAGGATCTCCAGGCGGTTCTCCAGTTCCTCCAGCTTGATGAGCTGCTGGTAGAACTTGAAAAAGTCGCCGCCGATCTCCTTCGCCAAGTTGCGGAAGAGGGCGTAGTAGATGCGCAGGTAGCTCACAGAAGTGATCGGTGCATGTCCCATCTGGACGCCCAGCTTGTTGGCAATCACGCCGGCCATGCGTTCCAGATCACCAGCGGTACAACCA

 

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