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CRIMIC CR02195

Gene CG13407

CG13407

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:22103414..22105590	94A2	FBti0213488	91468

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTACGTGTTACGAACAGTGATGGG	GAGTTTGTGCCAGCATACGGGTTA

Images

Construct Design Sequences

sgRNA sequence	GGGGGGGGGGGTTGGGCATGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCAGCACCACCACGATTACC	ATGTGGTTGTGTGGCCGAG	CCCACTCACTCATCCGCTTG	TGGGGCCGATAATCGTGATG

 

Amplicon 5'

Amplicon 3'

TCAGCACCACCACGATTACCACCCACTTCAAGTTCACCTGCGTTAATAGCCGCCTGCCAGGCAAGTGTCTGGGAAAGGCTTTGCCCAGTTGCCTAAACGACATTGTCACTTGCGGTTGGTTGGGCGCATCATCTCCTGCTGCTTCAGCCCCAAACTCCAGTTCGTCGTGCTCGTCCTGTTAGTCCTGATCGTCCTGTTTGCGGCTTCTGCAGTCGAGTTGCCATCGGTTTATGGCAGGTTGAGCACCAGCGACAATGAGCCACGCTGGTGCACCGTGTAGTGATAAACATGCGCGCTTTTCGGCACAAATCAATTAGCAATTGAATTTGGTGAACTCAAAGACGGTGAAAATGTCCGACGGCCAAATGACAAATCAACTCAACGTGCAACAGCAGGAACAACATCCGCTGCTTTCAGCCATCTCAGGAAATAATTATGCGTAAGCCATGGTCAAAGGCATTGGGATGTCGACTGTATTTGCCATACAGCCAAATCAAATTAAATTTGATATAATTAAGAAGTGGCACACTCACTCAGTTCCCTAGAATTATATTGTTAATCTGAACTCGCTTAAGTGCTTTTTATATATTTATCAAGAAGTCCTGCTAAACAACATCGTAAATAATGAATTAATTCTTTTTCAGTCATTGATATATGCAAAACTTAAACGCTTTAACAAACTTCTTTCTTCTATCCTACGTAATATTAATAATCGCTTAAAGGAGCCTTGAAAGAGTTTAGTCAGTTGCAACAGGATAAAGTGTGCTCTTTGGCTTAAAGATTAAGTGCTGGAGTGCCGGAGGCAACTGCAATCGCAAACGACCCCTGGCCCAAAAGACAGCCACTGCGGGCGCGGACTGATACCTCAGGCACTTAGCCGGGCTAATGTGGGACACAAACAGAAGCGGCGTCTCGGCCACACAACCACAT

CCCACTCACTCATCCGCTTGCCCGCTTTCACCACTCACCTTCTAACCTTCTCTTGCAGTCAAACGCAGCGTGTGCATTACTATCCCAACCACTACTACCCGAGCCACTACTTGCCGGGCCATTACCACCAAGGACACCAAGGATACCAGGGGCAGCAGGGACGACCACCGCTCCTGGCGGCCGCCAGCAGCCACAACGGTGCTTATGCGGCCACGGCGGCTGGCAGCGGACACTCCGGCTACGGGTAAGCAAAGAACCACCGCTGCACTGAGAGAAAAGCAATGGGATGAACAAGAAAATTCATTCCAAATATACAAAGAAATTAAACTGTTATGAAAAAATATAAATAACTATTTGAAGATGAATATATACTTAATAATATTGATACTAATGAATACTAAAGTTTCGAACTTATCAACCTAAATTACCTATTTCTTCCTGTGCACTGGCCATCAAAGTCAAACCATAACCCCAATTCCAGTGCAAGTTCCGCATCTAATGCCAGTTTGAATTTTGCAGCGGCATAGAGCCATCCGTGGAGTACGAGCTGCAGCATGACCCACCCTCGGGTAGCTTCTCCTCCTTTCATCCCAGTAGTGCGGGCAGTGGGCCCGCATATCCATCTGCCCACCATCCGCCGGCTGGTCCACCACCGGCTCCTCCGCCCACCAAATCGTCAAAGAAATCGAAGAAGAGCAGCGGTTCGGTGATGAACGCACTAACGCTGCTCTCGTTCTTCTTCTTTGTTAACATGCTGCAGAACTGCCTGAAGGATCATATGGCTGACATGAATCCCACCGTGATGGTGCTCACAGCCAGCGGCACTCGCAATCGTTTCAACAAATTAGCCGAGATGAATTCACGCGAACAGACCTCGACAACGGCCACCGAATCGGTAACGGCATCGGCAGCGGCATCATCTTGGAATCATCAGGCTGCTTTAGTTCCGGCCGTCGAGCCAAGTGTGCCGCCCTCCATTGTTACGCCCACGGTGGTGCTGCAATCGCCCTACAGCCGTCACACGGAGATGGCCAATAAGCCGCCCCAACGCCAGCCAATGGAGTACCAGTCGAACCCTCAGCAAAATCATCACGATTATCGGCCCCA

 

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