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CRIMIC CR02196

Gene CG2616

CG2616

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:7722417..7724715	84D13		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short	OK	GGAAGAGCTAACGATGGAATGGCA	CATGTGTGGGAAATCCCAGCACAA

Images

Construct Design Sequences

sgRNA sequence	AACACAATAACAGCTAGCTGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAGGAAGACTCAGGCGCATC	TCCATTTGCTAAACTCTGCAGC	TCACAGAGAACTACCTGGGGA	CCGGAGACTTTAAGCTGCGA

 

Amplicon 5'

Amplicon 3'

AAGGAAGACTCAGGCGCATCCAGTGCAGAGGCGAATCCAGCTGAATCGGAAATTTCGATGGGTCGAGGACCCCGCCCAAGGTCCTTTACTGGCGGCGGAGGCGGTGGCGGAACAGGTGGATCAGGTGGAAGTGGTGGTACCTCCGGCGGCAACAATCTCAAGCCCGAAAGAAGCGCCCGGGAAGATGACGCCATCAACCGTCTGACGGACTCGAAGTCGAGTCACCGAAAGCTGCTCAGCGATCCGCGATTCCAGATTCGGCCGCTGCAGCAGGTAATCTCGGCCTGCACCGGGGCCATGATCACGGCCTGCTTCATGACGCCCCTGGACGTGATCAAGACTCGCATGCAGTCGCAGCAGTCGCCGGCCCACAAGTGCTTCTTCTATTCGAACGGGCTGATGGACCACCTCTTCGCAAGTGGACCCAATGGCTCCGAGTTGGCGAGCCTGCGGCAAAGACCACAGTTTTCCAGCTCCTGGGATGCTCTGATGAAAATCAGCCGGCACGAAGGATTGGCCGCTCTTTGGTCCGGCCTGGGTCCCACCCTCGTCTCCGCCCTGCCCTCCACCATAATCTATTTCGTGGCCTATGAACAGTTCAAGGCCAGATACCTCCAGATTTACGAGAGCCACTATAATAAGAGTCAGGAGCCCCGGCACCTTGAAATCAGGTACTTACGTAACCCTTTAAATACATAGTGTAATACATACATGTAGTTTACCTGTTGAAGCGCTCATTAAGCAATTCTTCATAGCGAATATTTACATATCCAGCGCATGAATAACCATTTAGTTTCAATAAATTGTAAAATCGTTTTAGCTTTAGATAACAAATAAGCTGCAGAGTTTAGCAAATGGA

TCACAGAGAACTACCTGGGGAATACTAGTTTAAAAATATTTATTTTTCGTTTCATTTCGCTATTTCAAATGAAAAACATCTAAATTAAGGTTTATCAATACACATGCACATACAATACACATATTTCCACTGTATTAGACTGGATAGAAAATGTTTTGATGTATTGTACGATTTATTCGCAACTTCACAACCTATATATAAACCTATATAAGAATATGATTTAAGGATTTACAGGTTTAAACGTTTTCCATACTTCTAGGGATACCAAGAAAAGTCTACCGTCGGTGGTTCCCATGATGTCCGGGGTGACTGCTCGGATTTGTGCTGTTACCGTGGTGAGTCCCATCGAGCTGGTGAGAACCAAGATGCAGGCCCAGCGCCAGACCTACGCCCAGATGCTGCAGTTCGTCCGGAGTGTGGTGGCCCTTCAGGGCGTGTGGGGCTTGTGGCGGGGCCTGCGTCCCACGATCCTCAGGGACGTGCCCTTTTCAGGGATTTACTGGCCCATCTATGAGAGCCTAAAACAGAATCTCGGCCATGGCTCACAGCCCTCGTTCAGTCTGAGTTTCTTGGCAGGCGTGATGGCAGGGACAGTGGCCGCCATTGTGACCACTCCTTTCGACGTGGTCAAGACACATGAGCAGATTGAGTTTGGAGAAAGGGTCATCTTCACGGACTCGCCGGCGAGGGATTTTGGAAAGAAGTCGACCTTCAGCAGACTAACGGGTATTTACAGGACACACGGCGTGCGGGGACTCTTCGCAGGATGCGGACCCAGGCTTCTGAAGGTGGCACCTGCCTGCGCCATTATGATCTCCACCTTCGAGTACAGCAAGTCCTTCTTCTTCCATTACAACGTGAGGCATCATAACGAGGCCCTACTCCTGGACAACCCGAAAGACACGACGGTCGAAGATGATGACATTGAGTAGAGAGATGGGTGGCTCCTTTGTAAATAAAGAGCGCATATTTTATGTAATCCCCGGGTGGCGAGGCGAATTCAATATCCCCAACGCTTTACGAGTTACGTGTTACGTCAATAAGGCGAAGCCGGGAAATCAATTTCCATTTTCGCAGCTTAAAGTCTCCGG

 

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