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CRIMIC CR02197

Gene gro

CG8384

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:26041736..26049911	96F10	FBti0213489	91469

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TATGTACGTGCGTGCGAACGTATC	CGTGGATCTGTTGCTGCAATGGAA

Images

Construct Design Sequences

sgRNA sequence	GAAGCGGCGATTGTGGAGCATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCACCTCGGAACTATCGCTC	GCATTGAGTTGCGGCTGG	CCACAATCGCCGCTTCCTT	GGAGGTAGTGAGGCACTGGA

 

Amplicon 5'

Amplicon 3'

CCACCTCGGAACTATCGCTCTTGCTCTAGCCGGCAACATACCAGCCTAACAACATGTATCCCTCACCGGTGCGCCACCCCGCCGCCGGGGTAAGTTCACCCGACAGTGGGTCCCACCTCTGACCCCCGAATCCGTGCTAATTGTGTTTATGTCTCTTTCCACCACGCCGCGCCATGTGCCGCCCACGTACGGAACACCAACCACCTCTATTCACATCTAAATAGGGACCACCACCTCAGGGTCCAATCAAGTTCACCATCGCCGATACACTGGAACGCATCAAGGAGGAGTTCAACTTCCTGCAGGCGCAGTACCACAGGCAAGTATCCGGTGGAATACTCTATACACGGCAATGTTTACATATGTAAACATTACGCGAATCATATTTACAAGGGATATACAAGGTTTATCATTATAATTGTGAATACAAGGCAAATCTTTACTATAAAGATTTGCAAGGAAATATTTGCCATCATAATGACACTCTCTGCAAATGTCATCTTTGTAATTGCTATGTCAAGAAAAAACTACATTGAATATTGGCCCTAAGAACTATTGGCCCACTAAAATTGCTATGATCGCAAATTTATGTCAGATAAAAGTAAAATTCTTCACACTTTCAAATAATCCAATGGCATAGTTTTCATGGGTTAAGCAATACGCAATTAGAGATTAGGCCCCAATAATTAAGTTGAGGTTAAACCTATTAGAAGCCTTTACAATAACATGCCCCAAAGAAATCACATTTTAAGCCTTAGCCTTAACCGCCATCGTATGGAAGTGTGCGAGTGTGAGTGTGGATGGATGCTGCGTCTGCGTTTTGCGAAAAGTAAAACAAAAAGCTGAGCAAACCGGTTTGGCGCGCCATATGCGCCGCTGTCATAGGATTAACCTGCGCCGCCCCAGCCGCAACTCAATGC

CCACAATCGCCGCTTCCTTGCGTTCTTGAATTCAGTTCGCTTCCGTCGTCCAACTAATTTGCCCGTTGATCACTTTTCCTGTGAATTCCAGCATTAAATTGGAGTGCGAGAAGCTGTCGAACGAGAAGACGGAGATGCAGCGCCATTATGTTATGGTAAGTCGTCATGATCCGCAAATTATCGAGATCCCTTAGCTAATGCCACGGCTTGCCTTCCAACTTTCAGTACTACGAGATGTCCTATGGCCTTAACGTGGAGATGCACAAGCAGACCGAGATCGCCAAGCGGCTGAACACACTGATCAACCAGCTACTTCCATTCCTTCAGGCCGACCACCAGCAGCAAGTGCTGCAGGCGGTGGAGCGGGCGAAGCAGGTGACCATGCAGGAGCTCAACCTGATCATTGGGGTAAGTCGATCGAGGACGGATGATGGATTCTGCGGCAGCAGCAATACGGTTTCAGCCATTCTCAGATAGACACTAATGGGGGCATGGCCGATTGGAAATTCGGTTGCCACACCGAAAAGGCAGAGAAATTTAGTTTGCCGGGTACTTTGTAATACAGTCTTTCAAAGAATAACAAATATCTTGAAAATTGTATAAAAAATAAGAAATCTAACGTATATATGAAACAGTACATATCATAAATGTGGCAACCGTTAAGTGGGAACTTCGTTTCGGTTTAGTTTGTTCCTTGGGCCGCATTTGATTTGAGTTTGTTTTTCTACTTCCTAATTTGTTATGATTTCGCATTCGACCAATCCAATCCAAACAACGTTGTGCAGCATCAGCAGCAACATGGAATTCAGCAACTTTTAGTAAGTTTGTTTCGTTGCAAGTTAATTTCTTATTGTTTGTTTACTGTTGCCGTCTGCCGCTGCATCCGCCGAATGTGCCACTGCCACAACTACAACAACAGCTCCAGTGCCTCACTACCTCC

 

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