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CRIMIC CR02201

Gene Gp93

CG5520

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:27948711..27952151	98B6	FBti0213490	91470

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACAGCAAGGAGCTGGAAACGAATC	AACGCTTGAGCAACCAAGTGTGCT

Images

Construct Design Sequences

sgRNA sequence	GCCAATATTATAATACTACGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGAAGAAGCCCAGGACTTCC	TCCTTATCTTATCAGCGCGGA	TTGGCACTGGTGACGTGTAG	GTGCGGAACATCATCACAGC

 

Amplicon 5'

Amplicon 3'

GGAAGAAGCCCAGGACTTCCTGGAGGAGGACACCGTGCGCGAGCTCATCCGCAAGTACTCGCAGTTCATCAACTTCCCCATCCGCATGTGGTCCAGCAAGACCGTCGAGGAGGAGGTGCCCGTGGAGGAGGAGGCCAAGCCCGAGAAGAGCGAGGATGACGTGGAGGATGAGGACGCCAAGGTCGAGGAGGCCGAAGATGAGAAGCCCAAGACCAAAAAGGTTTCGAAGACCACCTGGGACTGGACCCTGATCAACGACAGCAAGCCCATCTGGACGCGCAAGCCCGCCGAAGTGACCGAGGATGAGTACACTAGCTTCTACAAGAGCCTGACCAAGGACTCCAGCGAGCCCCTGACCCAGACGCACTTCATCGCCGAGGGCGAGGTGACCTTCAAGAGTTTGCTCTACGTGCCCAAGGTGCAGCCATCGGAGTCCTTCAACCGCTACGGCACCAAGTCCGACAACATTAAGCTGTACGTGCGCCGTGTTTTCATCACCGACGAGTTCAACGACATGATGCCCAACTACCTGAGCTTCATTCGTGGCGTCGTCGACTCCGACGATCTGCCCCTGAACGTTTCCCGCGAAACTCTGCAGCAGCACAAGCTCATCAAGGTGATCAAGAAGAAGCTGGTCCGCAAGGTTCTCGATATGCTGAAGAAGATCGACAAGGAGGCGTATGAGAAGTTCTGGAAGGAGTTCTCCACCAAGTAAGTGTCGCTTTCTAGGAATTAAGAAACGTTCTTTGCCTGTTCAAATAATTAGTTATTTATGATAAGCATTTCCGCAGAATCCATTATAATATGGAGAATAGAATGACGTGTATTATTTTTTGCCAACAAATTAATATATTTAGTTTGTTGCTTTAAAGAATTCCGCGCTGATAAGATAAGGA

TTGGCACTGGTGACGTGTAGAGTTTATACTTAAATATTTTATTTTAGTTAAATAAGCATTTCCATTAGCTATAACCTCGAATTCTTGGATTTTCGTTGCTTATCAAGACTTAATTGACTCATTTCAGCATCAAATTGGGCGTGATGGAGGATCCCAGCAACCGATCGCGTCTCGCCAAGCTGCTCCGCTTCCAGACCTCCAACGGCAAGGGCGTCACCTCGCTGGCGGAGTACAAGGAGCGCATGAAGGCCAAGCAGGAGCACATCTACTACATCGCTGGTGCCAACCGCGCTGAGGTCGAGAAGTCGCCGTTCGTCGAGCGTCTGCTGAGCAAGGGATACGAGGTGCTGTATCTGGTGGAGGCCGTCGACGAGTACTGCATCTCCGCGCTGCCCGAGTTCGATGGCAAGAAGTTCCAGAACGTGGCCAAGGAGGGATTCCAGCTGAACGAGTCCGAGAAGAGCAAGAAGAACTTCGAGTCGCTAAAGAGCACCTTCGAGCCGCTGGTCAAGTGGCTGAACGATGTGGCACTCAAGGATCAGATCTCCAAGGCCCAGGTTTCCGAGCGTCTGAGCAACTCGCCATGCGCCCTCGTGGCCGGCGTCTTCGGCTGGACCGGCAACATGGAGCGTCTGGCCATGTCCAACGCCCACCAGAAGTCCGACGATCCTCAGCGCACCTACTACCTCAACCAGAAGAAAACGCTCGAGATCAATCCCAGGCATCCGCTGATGCGCGAGCTGCTGCGCCGCGTGGAGGCCGACGAGGCCGATGACACCGCCAAGGACATGGCTGTGATGATGTTCCGCAC

 

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