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CRIMIC CR02203

Gene CG31121

CG31121

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:24881466..24894605	96B2-96B4	FBti0213492	91472

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAAAGCATTGCCGTAATCCGAGT	AACCTGCTGTCCGTTGATGAGGAT

Images

Construct Design Sequences

sgRNA sequence	GGAGTAGCTGGGGTAACCACTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGACCGTTTCCATTGCCGTA	TGCCCATCGAAGTTTTGACG	TTGCTGTTCCTCCAGTGACC	GGCTGGCAAAACGGAAATCC

 

Amplicon 5'

Amplicon 3'

TGACCGTTTCCATTGCCGTACTGCCTGGAGCTGCCGTACAGTCGGGCTTCGTTATAGTCGTAGCCCATCCCACCACCGCCGCCATCCACCTGGCTCAATCCGGCCCCCAGAAGATCCGCCTCGCTGTGCGATCTGTGACCGTAGTGCTTCCCGTACTGCTGTTGATGCTGCTGCATCTGATGGTGGTGCTGACCCACCGCGATGCTTGCTCGGCTGTTTGCCTGCCGCAGGGCAGCCATCGGGACACCGCCTCCTCCGATCTGAGCCCGCGACTCAGGACGCGAGTGCGGCATGCCGCCGATGGCACGGAAGTCGGACTCGCTGCGATACTTCCGGAGATTGGGCGGCACCCGACTGCGGGTCGTCTCGTTGTCGCTGCTATCGTAGCCACTGCTCCCGGCTCCAGCCGGTGCACCCGAGCTATCCCGAAAGGCGCGGTTCACTCGTCCTCGCACTCCGCCCAGGGCACTGGAACCACCATGGCCATGACCCGGTCCCGCACCCGGACCCGGACCGGGACGATGCGATCGCTGCGATCCAGCATTTGGCGGAAAGACGCGCGGCAGTCCGAACTTCAAGTATGTGTACATATTTGAGCCCAAGGGAGATCTGAAAGGATTAGGTATGGTCGGATATATAGTATGGTGATAACGTTTAAATCTTATGCTTAAATATATAAAGGTATAGCTAATTCAATTAATGTGTTTTTTGTACGTAACCGAAATTCTCAAGTGCTGAGATCATACATAGTCCGGAACTATTAACGCTTGATTATGCTTAATAATTGTTTATTTAAATAATGTATGAGATTACTCACTTGGGTCCGTAGCGCGGATGGTTTAAGATTGATTGAACTGTGGTATCGCGTAGCTGGAAGTTTCCATATGGCAGCGGTGATTTGTCCGACGAGCCCAGAGCTGAATCGGTGAAGTCAGAGTTCAGGTTTTGCCTACGGATGGGATGGGATGGGGGTATTTGTATTGCTCGTTGTGGAGGATGGAAAGCGTCATAGAGAGCTGCACTGGGCGGCTGCAGTTGCGGTCAGCTGGACATGATTGGTGGGCTTGGCCGTGGGCTCTTCAATTAGCCATTTATGGCATTTGAACTTCCGTCCACTGCGGTTAAATCAACCGTCAAAACTTCGATGGGCA

TTGCTGTTCCTCCAGTGACCACACCCAGTTCTCGAAATGGGGTTCCTCCACCGTTAACCACTCAATCACTCACCTGTAGATGGACCAGGCATGCAGATCCTCGGATGCCGCCGGTGGCAGTGGGGTATTGGGTCCGTGGGGCACCGAGTAGCGGCGCTCCTCGCGTGACATCTTCTTGCCGCTCGAAAAGTTGCTCAGCTCCATGATCTGCCGGAAAGCACACGGATCGGGTTAGAGAGTTCGGGCCAAAATATTAAGCTAATTCCGACTTAAAGTGGGTCAAGAATGACGCGAGAATGGCTGCGCTATTATTACTCATAATTGGCGGGAATTAGCCTAATGACAATGCCCACACATATCCACACGCAGCGCCAGTTGGAGTCGAAGTTGGATTCGGAATCGGAGTCGCAGTCGCATATTCACAACCATTGTCATGACAAAAGCGGAAATGGTGTCGTATATGAGGCGAGGCGAACTTAAGACACCCTAACAAAGACCTTTTCGAAAATTTGATAGATACTTTATCTACTTTTCATAATAAATAAATCAGAAAAAAATAATGTCTGTGCTGTATTTATTAACTTACTACCCATGGGTTCTTTTGTAGTTGTGCAATATCATATATATTTTTTGTGAGTCTTAATCCGAACAGGTGCACAAAAAGCCACCAAGACAATGCTAGCTAATCGGGTGTTGTCTTTCAGATTTCCAGCTCCGTATCTTGAGGAAATCCAAAACACCGGGCGGTAGAACAAAGCGCAGCTCATTTGGACACGGCCTCTGGGTTTTTAATTGCCAATGCCCGCCGAAAAAAAAAGGTAGCAAAAATCCATGCAAATGAACCAATGTTTGCTCGGCAGCATTATGCAATGTTAAGCGAACGAAAGGCAAACAAACTAATGCAACTGCTGTCGCCGGATTGTGTGGACGCCAAATGGATGCAGTGCAGCGAGCCGTGCGGATTTCCGTTTTGCCAGCC

 

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