• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR02204

Gene jagn

CG10978

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:5779315..5781308	83C3	FBti0213493	91473

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
		TGTAGTTCCTGAGATCTCGACGTG	GAAGTAACCATCCGTGGACGCAAA

Images

Construct Design Sequences

sgRNA sequence	CTGCAGTAGGTGCCGCCTGCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTCATCGCGACCCGTTCATT	GGTGGATCTACTGCCACAGG	ACCTACTGCAGCCGAATCG	TCACAAGGCTGCGTATGTGT

 

Amplicon 5'

Amplicon 3'

TTCATCGCGACCCGTTCATTAATCATTAATCATTACTAAATCTTACTAGTTCTGCCATTTGGTCTATAAGACATATTGGAATTGGAATGCAATTCAATTCATTTTAGTCTAGCGTTTGTACTTTTAGTAGAAATAATTCTCATATGGATGGAAGATTTCAATCGGAGTATTAAAACCAGCCCTCATTTTCTAAAATCGTGCAAAAAAGATATGAAAATTAAAGTATTAAGTGCCATTAATGCCATTAAGAATAGGTTTGGTTTTATTTTTCACCAGCCCACTTTTCAGATTAGCAATGCTAAACTCTCAGCATTACCTTTAAAAAATCGTTCTCATTTGCGACTAGTTCGAATATCTATCTACATTCTGCCGAAACTAACTAGAGAAAAACTGAAGTTCTACGTATGTAAGTATAACACATTTATTCTACACAGCATTAGCGAGGTTACACACCTAGCGAAGTCATCCTCCCAATTAAAATTTCCCTAAGCGGAGCTAGTCACCACGACCACATTGCCACTTTATTAAAACTAATAACTGTTTAATAACTGTAATAGAATAAGTTAAAATAATTAAATAATATGACTAATCACGTGTTGCTAATACAGTTAAAAGGCTAATTATTTAATTTAATTAATGTTTTGTAAACAGTTTCCATGCAGATGTATTAATAGTTTCCATGGCAGACTTTCTGAAAATGAACATTCTAAAACTTAGCGGAATTTCCCCTCTCTTTCTAACGGTTATTTGCTATGCTTTCAAGCGAAAGTAATCGATGGTAGCGAGGAGCGTATCGATGTGCGCCAGTTTTTTGGTGGCAGCGATGGTATCGCACACACATCGATTCTAGTGCCAGCCCTATTTTTTGTGCAATTATTTATTAAAAATAAGCAACGACGTGAGCGAACGACACGACCCACGAACGAAGGCAACGAAATGGCAACGCGCGGCGGACCAATGGTCGCGGGCACCGATGGCAACGACTTCGAGTTCAGGCAGCGCGTAGCTGGCACATATCAAATTAGGTTAATAACACTTCGTGCTATCGCCAGCTCTCAGCTACACTCGAAATTATCTAATTACTGGGGCGCCTGTGGCAGTAGATCCACC

ACCTACTGCAGCCGAATCGCAGGTGGCGTCCAGCCAGGGCGGCGGAGCACCCTGGAGCTGCTGCACCCACCCACTTGCTGCTGTAGGGGGCGGGTGGGCAAAGTGCCAGCTGGGCAGCATGTGCGCCCCAATATGGGGCTCACCCACACAGATCCGCCACTGAACGACCACTTTCTCTGCTCTTTTAGCCTGCTGAACAAGTCCAGGTTGAAGTACTGCATCTTCTTTCACGCCCTGCTGTTCTTCGTGATGCTGGCCAAGCTGACGTCGGACATTCTGGACCACCTGGATATCTTTGTGCTGGAGATCGAGGAGCTGGAGGTCCCACCGCCCCTGTGGTGGGAGTACGTGTGGGCCGCGTCGTTGCTCACCTCCTTCCTGGGCCTTTCGGCCGCCAGGGGCAACAAGGTGCGCGAAATGCAGAAGTATATGGTGGCCATACTGCTATTCGCCATCCTGCCACTCTTCTACTGCTTCGCCTACTACTTCTCGGACGTGTGGGAGTTCGCCACGCTGGACAAGTCGGTGGAGCTGGACGAGACAGACATCTTTGTGTGGCGCGTAAGTGGCTGGGCCAGTTAATCCCTCAAGAGCCGCCCTACTAAACCTCGGTTTTTCTACAGGGTTATCCGTACGGCGTCTTCTGGTATGCCTTCTGCTTTGTGGGCTTCCAGGTGCACGGATTTACGCTGTACTTCGCCTACAACCTGGTCAAGGCCTGGAAGGCCAGAACTGCCACGCGCAAGTTCCAGTAATCTAAATACTCGCCAAACTAGTAAACGCCGGAATGCAGGGCTAGTTGTAAATAATTTGGAAACTTGAAACTAACTCAAAACACTAGTGCACAAAAGCGTAATGAAACTATGGTAAACTAAAGTTATGCAGCTGTTAAGGTATCGCACACTTAGTCATACAGGAAGGGAAACATATGGTCGAAACTGAGCGAAGCGTCGGCGGTAAGGTTATTGTTAGTTGAAAGTTAGTTGATACCGTTTTCAAATTGATTAAATAGACTCTTATCCATATACACATGTACACCTATACACATAGACATACATATATACACACATACGCAGCCTTGTGA

 

---

Search Again