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CRIMIC CR02205

Gene CG13300

CG13300

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:6329324..6352390	65A14-65B1	FBti0213494	91474

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCCCACTCCATTCACCATGATCC	GACTCAACTGTGGCATACCCATTG

Images

Construct Design Sequences

sgRNA sequence	AATGTTGTGAGAGTCAACACAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTCCCTGGATGAACTGGCAA	TCGTCGACTGTTTTCGCAAC	ATAATGGCTAACAGCGGGCA	CAATAAGCGAGGAGGAGCGT

 

Amplicon 5'

Amplicon 3'

GTCCCTGGATGAACTGGCAAAAATAAACTTCACAAAATTGTTACGTGTAATCCCCTTTATGATCTGCACCCATCCATGTGCGCTGCATCTTAAGTGAAACGGGTCCACAGACCCATTCACATTACTGCTGCCAAATCCAGGCGCAGTCCTGGCCCGTTTCTCAGCTGGCTATGACCATGGTGGCTGATGTGGCACCCGGTGGCCAGTGGGTGGCTCGGGAATTGCTTGCCTCCTGTGGCAGCGATGACGATCCCATCGAGGACAACGAACTACTGCTGTTGACCGAGTGCAGCTGCTGCGGCTGATGGTACTCCGGTTCCTCGTAGGCGGCATCCTCGTCACAGTCGTGTGGATACAGCGGCACATGGTGGTGCGTGTGATGGTGGTGCGTATGCGAGTGGTGCACATGCTGTTGCTGCTGGTATGGATGGAAGTACTGCTGCTGTTGCAACGGCTGATGTTGCTGCTGCTGTGTAGCAGGCGGTGTGGTCGACGACGACGCTGGCGAGGCTCCACCAGAAGGTGGGTGGGGCAATGACAGTGGCAGAGGCAGCACCACCGGCAGCGGTTGCAGACCTCGTATGCTGTCCGCCGGGTGGGCCGTGTAGATGTTGCTCCTCCTCAGGTGGTTGTTGTTTATGTTGCTGCTGCTGCCGTTGTGGTGGTGCACATTGCCGCCGGCACCACCCGCCGCTCCACTTGCCCCGCCCGCGCCATTCAATCCGTTGCCGTGGGCGCGTTTGTTGCGAGACCACCATTGCACGTGCGTGTCTGCGTGTTGGAGAAACCGATGCAGATGCCACACAATAACAAACACGAGTGCCGAAAAATAGAGAGAAGAGAGGGTGGTGGAGAAACGGGAGGGGGTTAGTTGGTGGGTCACAGTTGCGAAAACAGTCGACGA

ATAATGGCTAACAGCGGGCACAGTGGAAATCATTCATCATAAAAGGGTTAAAATAATACAAAATGTTTCACATTTTAAGATTTTACTTTGTAAAGCTTAAATCTTTGTATTTCATTTGGTTAAAAGTATTATTTTAGATGTTTTATTTGCAACTCTGAATGATTGATTATCCACTACGCCCACTGTGCTTGTCCTACGCCCACTGTTTTCTATTTTGGCCAAGTTGGCCATGTGGCTATGTCGAGTATTAGCACTCACCGTTGCGATTGTTGGAGGACTTTTTGCGCCGGCAGCGCACCACGGCAATCATGATGATGGCGCCTAGCAGCGCCGACAACACGGACACGATGATCAGAGTCAGTGGCCAGCCCAGTTCTGTGGCCTTTTCTGCAACAAAAAAAGGGGATGGGCAAAAAGGGTAACGGTTAGTAACGGAAGTTATGCAGCCCTCAGCTACCAATTCTGCACTAAGAAAAAATGTATAAAATATAATACAAATTGTATACGTTTTTTCTAAGTCTTTTAGATTGTCTAGCCAAAGCTCAATATTTAATTTTTAAATGCATATGTATCTATATTCTTTTTGTGCCAGTCAACTTTAATCAGAATATAAACCTCGAATTTGTATCCCCCAGTGCATCCCGACTTGTATTCGGTTACTCACCTATCGTTCCCTCGAACATGAATGTGCTCTTGGTGGGCATCGCCCAGGAGCACAAATCACACGTGGTCAGGCCGTCGCTCAGCACCGGCTCGTCGACGAAGAAAGGCGGGCGGGGCGGTGGCGGTACAAAGCGTCCGTTCCAGAGGCCCTGTTCGCTGGCATCAAAGGCCATTGGCGAGCCGAACAAGTCGTCATCGTCATCGAAGATGTCGAACGTCACGTCACCAAAGCTGCTCAAGTCCTCCGCGGAGCTGCTGGCCACCAGACTGGCAATCCGCGGTGGACTGAAAGTAGGAAATTCGGATGCGGATACGGAGGGCAGGGCGGATGCGGATGCGGATATGGACACAGATACGGATGCAACTTGATTTACAACGTAACCGAGACGCGCATCGTTGCTGTAGTTGCTGTTTGCTACGGATGGCATTTTCCTTGAGTTTTCCGGGTGGGGGATTGAGGGAGTTTTCCGGATAAAGGGGCAAAGCTGGAATCCTTTGACGCTCCTCCTCGCTTATTG

 

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