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CRIMIC CR02206

Gene sals

CG31374

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:11437547..11451791	86E5-86E6	FBti0213495	91475

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GACGATCTGCAGAGCACCAAACAA	CGCGACATCGATACATACATCGAC

Images

Construct Design Sequences

sgRNA sequence	GTCTGGGCGGAAGCAGAATGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AATAACCGCGTCCAGTTCGT	AAAGCGGTGTTATGACCCGT	GGAGTCTTTCATGCAGCATGC	CAAGACGGAGATCGAGGAGC

 

Amplicon 5'

Amplicon 3'

AATAACCGCGTCCAGTTCGTCCTGCAGATTGGTGGCCCGCGATCGCACCTGCACAATTTGATCCTGCAAATTGTCGACGTTCGCCTGGAGCAGCACATTGCACTTCTTCATCGCCGCGAACCGGCTCTCGTGCTTCTTCACGCGCGGCTCCACATTCGCCTTCTTCGCGGAGTTGGGCGAGTCCTGCGGAATACGGTACAGGTGAGCTGGCTAAATAGTTTACGATTCTCTGGGGCTAACTCACCTCTGGCTCGGACTCGCTGCCCGTCTCCGCCTCACTTTCCTCCGATTCGGACTCCTCGGACTCGTCGTCGGATTCCTCCTCTTCTTCGCTGGCGTGCTCCTCCTCGGCCTCCTCTTCGTCCTCGTCCTTCTCCTCGCCGTCCACATCGTCTTCGTCTAGCTTCATGGAGGCAGCCATCTTCTGCACCTCCTTCTCCAGCTTCTTAAACTTCTTGTTCTCCTCCCTGTCATCCAGAGTTGTTTCCAGTTTAAGAACAATACTACATTACAATTTGTACTCACTTGAGGATACTCTGGTTCTTCTTCATGGCTTGCTTGAGGGCGTCCTTCTCCTTCTTGGCCGCCTCCTCTTTGACCTTCAGTTTGGTAAGCTTGCTACGCAGGATCTTGAGCTCCTTGCCAGAGCGACGCTCCCTGCGCTCCACCTTCTTCTCCTCCGGCTCGTCAGGATCGCTTTCGCTGACCTCAGGCTCCAGTTCCGGCGAGCTATCCCTTCCAAAGTTTGCATCGCTTGGTCGACGCGATCCGTCCAAGGATCCACCGCCCTGCAGCCGCAGTTAATGCAAAACGGAAAACGGGAAAGAAGGGATACACACATTGGATTAGTGAGGGCATCCTTGAGCGGGGCCTGATTTTACCTGATGCCGTGCTGCATGGTAGTGGTCGTAGTGGAAGCAATCGTCGTAGTCGTCGTCGTCGTAAGCAAAGCAATTACTAATGTGCGGTCTGTTAGTGCTTGGTGTCGCCTTTGCAAGAGAGCGTGAACGGGTTAATCGGTTAGTTGCCGCGGAGTGCAATAGCAAAGCTTTCAGGGGGATAGGAATCGGGACCGGACGTGTCCTAGACGGGTCATAACACCGCTTT

GGAGTCTTTCATGCAGCATGCGTCCGGCAGTTGGAGTTTAAGGTCGTCTACACACGAGATCTTCGGACATTGTTTATACCTCTCGTTTCGTTTTGATAAAGATTACATTTTTTAATGTCTACCATGGCATGGCCAGGTCCATTATTTGCTTAAAGAAAAATTCTGGAACCTTTTGAGCATTTCCCCAGAAACTTGATTTTGTTTTGTCTGGAATTGTGTGCAAGCAAACAGATAGCACAGGTAAGGGATGAAGGCACCTTACTTGAAAAGTATGGCTTCCGAAATTTAATACAAATTGAGCTTTGATAAAATATGGTCAGTTTTGAATTTCATTTTTCGCTTTCTCTTGATAATAATTTTACTATTCAACTTGTCTGATTCCTTCTCAACAGCAAGCTGTCTGCTGGCTTCAATCAGATTTCTGGTATTTTAAGTCTCGTGTGCAAACCGTTTTTAAGAAGGAAGAAAACAAGGTGTATCCACGGGTAATCCTTTCCCAGACCCATTCCACATTTGTGTCAAGTGTTGTCAGCTGATCTGTTTCTCTGGCTACAGTGGCTACTAGGGACAACTAACGAGGGATCTAAGGCGGAGTCTACGAATTGCGGTGGCTTCGTTAGCTGCTCCCGTCTACTTGCCGCCCTCAGCGGACGCAGCGAGGCGACGCCGGCGACACTAAAAACAAGAAAGAAGGACTAGCTTCGGAGTCCGATGCTTTGCATACCCTTGGAGATAGTTATGTTGTAAATGGTTGTAATCCAAAATCCTAATCTCAACTGACAACACTTTGAGCAGCATCCCATTACCTACATATACATATTACCGAACCCGTTCTGCAAAAGGAATTACCTGAAGAGCTGCTGCCAACTTGCGTTCGAGCAACTGAATCTGGCGGCGCTGCTCCTCGATCTCCGTCTTG

 

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