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CRIMIC CR02208

Gene CG14238

CG14238

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:26458249..26460981	97B3	FBti0213496	91476

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGTCACACAACGCACATCATCCT	CGACGAGATTGAAACTGACGTGAC

Images

Construct Design Sequences

sgRNA sequence	GGGTGCGGCCGCAGTGTTGGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GACTCCGCGTTTATCGTCCA	GAAAGTGGGTGGCCAGGTTA	CGCACCCCCACACTCAAA	CACTCCTCCTTGTGATGCGA

 

Amplicon 5'

Amplicon 3'

GACTCCGCGTTTATCGTCCACCGTTTGCCGTCTTAAAAGTCAGCTGAAGCATTTAATGGTCGTGGTGTGACTTGGAGGCACACATAAGTGAAACACGCATGGGTTCGATTTCGGTTGGGAAGACATTTCCAGGAGCGTCTTGGTCACATCAGAGTATAGAAAGTAAATGAAACTTAATTTCCCATATATTAAAATGGAAAAAAAAGGTTTTAAAATATATTAAGAAAGTTATTATTAGCTAGAATTTATTTAAGATCCATATTTTTTACTCTCTCCTTACTATGAAGAATCTTTACTTTTCGGTCTGCAGTTCGTCCCTGGACAAGCCGAAGATGATTCGTCCTGAACGCACACACAGGACATCGGGACGGGGGTCCGTGGCCATTCACCAATTACGCGCCATTCGCGGCTGTGTCCTTAATTTCTTAAAAAGCGTTTAAACATCTAATGAAAGCATTTGGGACCCCGCGATTTCTGCTGTCAGTTGCGTTTACCTTTTCGCCGTGTGCGGTTCGCTTCGTCGGTTCGGGTCGATTTGGTGGTATTCCTCGGTCCTGATTATCCGAGCTGCAGGAAGCGAGCACGAGAGGATTATGTCATTGCAGCCATGGCCAAGATAATCTCGACGATCAAGGCCATCCTCACGAGGATCATCTTCAGTGCGCACAGCCTGCTGGCCATTTGGCAGGTGGTGGCTTTAAAGAGTGATATCATATACTGGGCACTATGTGGACCACTGCTGTTGCTGCTGCTCGAGGGCATCTTCACCATCATGATCAAGAAGACGCAGGAGTGGCGCTGGTAAGGATGAAACCCACGGATCCTGGCTACCCCAGGCATCTGATTAAAACTCCCAACTCGAAATGTAAACAACAGTCAGTCGATGCTGACGTCATGCCGCGGGGTTTTGATTTTCAATTAAAAGTGAAATCATGTGTTTAATGTGTGCCTAACCTGGCCACCCACTTTC

CGCACCCCCACACTCAAAAAAAAAGTAGCCACGATTTCCAAACAACACAATGTAGTTTACATAGGATTTTTGGATAAAAATATGCATATGAATTGAATCTAGAAATAGGAAATCCTATGAATTTTGTATCTTAAAGCCAATTTGTATTATCATTTCGGGGTGTACTTTTTTTCAAGTGTAAGCTGTCCACCTCACACTTTGTACTAGTAAAAACTTTAATGGAATTTCGTGGGGGATGGAGTCTTTTTGACTCTTGTTTACCTTTCGTCGCATCACTCAGACTTGTTGTTTGTTTTGGCCGTTCGGCTGTGCCACCTCATTTGTGTCCTTTGTCCGCAGGTTCTGCCCCTCGGTTTTCCTCTACCTGAGCAGCATTGTGCCGGCGATATGGCTGCTGGAGCTGGACAAGGTAGCACGTCGGGTGGGCAACATGACATCGACCAACTCGACGGTCTACAGTCCGAGCAACTACAGCATTCCCGTGGGCAACACCACCATCTTCATCACCAGCACCAACAGCAGTGGCGTGCCACCGGTGGCCACTCTGCTGGGTCAGGCCGGGGTCACCCTCTCGGTGACCCCGGACACTTGGACAACCCTCATCGAGCAGTTCCTCATGCTCATCCTGATCGTTGGTCGCTGGCTGCTGCCCAAGGGCGATCTAACCAGGGATCAGCTCAGCCAGTTGCTCCTGGTCTACATTGGCACGGCGGCGGATATTATTGAATTTTTCGATTCCTACAAGGATGACTCGATAGCAAAGATTGGATTCCTGGTCTTCCTTACACTGGGCATATGGTCCTGGAGCCTCATGCAGTTCACCATCGTGCTGTCCGCCACTCGAGGACGACGACCACGAGGCAGTGGATCGCATCACAAGGAGGAGTG

 

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