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CRIMIC CR02212

Gene beat-Vb

CG31298

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:12916056..12934813	87D4	FBti0213497	91477

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGCGCTTTGCTGAGATTAGCATC	GCAGAGGGAGAAATGAGAACAAGTGC

Images

Construct Design Sequences

sgRNA sequence	CAGGACACAACTGTTGTTTGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACTGCTTTTGGCCCTTGACT	TGCGGTTGTGTGAGTGTG	GTCCTGTCTTTTTAGTCGCGC	CAACCGCGTGCTGGTTTTTA

 

Amplicon 5'

Amplicon 3'

ACTGCTTTTGGCCCTTGACTTTCATTAGTACTGGCTGAGATGTCTTCTTTCTTTTCCGAGCCCAAGAGCGAACCGGCTCGCCTGACAAGCGGAATGGGAAATCATCACTAAGGAAAATTATAAATAAAACAGCAGATAGCCAGAGTTCAAGATTTTTTCACTCGAAAACACTTATTATATAAAGAAGATATTCATTTCAACTATATCAAAACACGAAATGATTTTGGCCATAAAGACCAAAAAAATTAAATTTATTTAGGAATATACTGAAAGATAATAAACAAGACAAATCATATTTTCATTAGCTAAAATTTTAATGTGTACGCAAAATATTTATAAAAACAAAAGAAAATTAATGAACAAACTATATAAAGCGATTATCTGGTTAATTGCTTGGTAAAATCTGGTTACTATCACTTAGTAAAAGTAATAACACAAAATAACCTGAGATTGCGATATGCTTGCAGGCATACGGTCATTTTGTATTGGGTAAAATCTTTAAAAATCCGTTTGGCGCGAAATTAAAGGTAGTAACAGCCAAAAAAAATTGACACTTCATAGAGGGTTTAAATAAAGAAAGACATTATTGTATACAGTTTTTTAGATGTGCTTAGTATTGCAGCGTTTTTTCTAGTGCACAGGAGCATCAAGAAATTGACTTGCAATCTTGTCTTGGTCCTGGTTTTTCCTCCGACTCGGATTCATCCTCAGTATGAGCCGCTGGTTTTGCTCCTGGCCTTCGTTGTTAGGCAGCAAAGTTCACTCAAGTGCGTTGATTGCCGTGCAAAAAAGTTTCGTTTGTCTGTATTTGATGTACTTATTTTTATTATTTACATACACAAGCAGTCGGCATCGGTACGCACACAACCATACACACCCAGACACACTCACACAACCGCA

GTCCTGTCTTTTTAGTCGCGCAGGTGAAGTTTTCCATTTACTTTGCCTCTACGGCTGTCATTAAGCTGCGGTTGCCGCTGCCATTTGCAAATGTGTTTGCCATTGTCGTCCTTGTTGGTGCTGCTGCTGCTCCTATTCTTGTTGTTCGTCGGCTGCTTTGACATGCTTCAAAGGACTCATGTCAGGGATTGGAAACCGAATCAGAATTGGAATCAGAATCACAGCCAGGCAGGCACTTTTGTCTGTTTGTCTATTGAAAGTTTAAACAAGTTTGTCTCGCCCGTTTTCCCATTTTCCAGATGTTGCACCAACTCAAAGAGTTTTTCCTCGAAGGACGAACTCGAGCAAGCCGAAAAATAAATACTCTGCTCGTGGTGCCTTCGGCAATTGCAACTTTTGACATCCTTGCAAGTTATTTCAACTGTAAACTGCGACAAATTAAATTCACGAGTGTTGCAGTTGCAACTGTTGCTGCAGGTGCTGTTGTTGTTGCCACTGCTGTTGTTGCTGTTGGTATTGCTGCTGCTGTTACCTAATGCGATTTGCGAAAATTATGCACAAGTTGTTGGCTTCCACTTGGCGACGTCTCGCGGATTTCTTGCTTATCATGCGGCAGTGTGCTCCAGTTGCAATTGCAACTTCAACTGCAGCAACTGACAGGGCCAGGATTATGGAAATTCTGTACTGGCAGCAAGTTCGACATTTAGCGTAATATAAGGAGATGGAAAAACACCGCGGCAACAGCAACAAAAACCCATTGCAGCTAATGATATTAACCATTTTTTCCGGCATTTTCCCCCTTTTTTTGCACCCGTTTCTCTCTGGCTCTGTCTGTTTGCACTTAAAAGCGTTCTGCAAATTGTAAAAACCAGCACGCGGTTG

 

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