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CRIMIC CR02214

Gene Ge-1

CG6181

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2L:11095150..11100888	32D3	FBti0213518	91490

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	agaacatcgtcgactacaagtgggag	aacagcgaactgacatcgatcacagc

Images

Construct Design Sequences

sgRNA sequence	ATAATTAAACCATCATTCCCCGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGTCCACCGCGATGGCAAGCACCTGGCGTACGCCATTAATGGTAAGTAATTTATATCCCATTGCAGTTTCTTGACCAATTAGATTTTATTTATGTGTGACAACTTTTTCTCGCAATTCTTGAGTTTTATAAAAAAGGAATGATTCTTGAATTTATCGATGGGAATCATGTCCCAATCAATATTTTACAGAGCTTCCGGGG	AATGATGGTTTAATTATTATGTATACTTATGAACCCATTTACCTTGTGTTAATTTTAAAATTAATTTAAAAGCTATTGTATAATAACTTCATTGTGCTCCCAGTCAACAACAAAGCCACCGGAATGGAGGGCATGGTGCGCGTATGCAACATAGCCACCAGTATGCGAGCCTTGATCAAGGGAATGAGCGGCGAGGTCTT

 

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