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CRIMIC CR02215

Gene trp

CG7875

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	3R:29914521..29920289	99C6-99C7	FBti0213519	91491

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGATCCTGCTGGAGCATAACATCGA	TCATCTGGGAGGTCACACACTCAT

Images

Construct Design Sequences

sgRNA sequence	AACGTGCCGCTCGAAGGACGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GAGGCGGTGGACCGCTCCAAGTCCACCTTCACCGTGGACATCACGCCCCTTATCCTGGCCGCCCACCGAAATAACTACGAGATACTCAAAATCCTCCTGGATCGCGGGGCCACGCTGCCCATGCCGCACGACGTCAAGTGAGTGGGGTGGTGTAGGGATGCAGTTGCTCGGTGATATGGCTATGTAGTGAGCTTCCTCGT	CCTTCGAGCGGCACGTTCCATTGTTTGTCCAATTGGCAACACACTTATGTAGCACATCGCTGGGTGCCTCTAATTTTAGATAATTGCGGGCAAATTAGGGTGAAATATTAATACAGGGTGTAATGTTGTGTGTGGGAAGAGCAGGTCCTTGAATGTTCTTAAATTGGGTCAATGGACTCAAGCCTTTAACAGCGTCATAA

 

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