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CRIMIC CR02226

Gene hang

CG32575

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	X:16422831..16438454	14C4	FBti0214874	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	PK	GTTATGGGTATGGGTATGGGTGTG	TTCTACTCCTCCGTCTCCTCTTTC

Images

Construct Design Sequences

sgRNA sequence	GGATGATGCCGAGTAACCATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGGTCTCCGATTCCGAGTCCGAGTCCGCCGCTCTCACTGAACGCTCTCTTGGGTGCTCTGGCACTCCGGTGCTCTGGTGGTTGGACACCATTAACAGTTATACACACAGATCCCAAATGGTCAGTGGAAGGATGAGGCGGTGGCCACACCCGTTCGCTGGACAGAATGGAAAAGTGAGGTTACGGATGATGCCGAGTAAC	CATTGGGCAGTGGGCATTGGGCCCCGGAGTTCAAGACCCATCGAAGGGGGATTGTGCAAGCAACCAAAGATGATGGCCAGCCACATCCAAAAACGAAAAACCAAGCAAACAAAAAAAAAAAAAACGAACGAACCGAAAACCAGCCAAACTCCGCCTCCTACTCCTCATCTGCTTTGGTATGGGTGTGTGGCTAGGTTTTG

 

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