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CRIMIC CR02229

Gene Spt6

CG12225

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	X:6267797..6274984	5E4	FBti0214875	92206

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTCACCATCCATCGATCGGAACA	ATCGGAATCGTAACCACTGCCATC

Images

Construct Design Sequences

sgRNA sequence	GGGAGAATTAGTTCAATAGATGG
sgRNA2 sequence

 

left_200bp	right_200bp
TACAACTTTGAGGTTAGGTCTTGTGTTTTTACCGTGCTACCGTGGCCATCAAATATCAGCATCAGCAGTAGCAATAGCAGCAGCAACAACAACAAACAGCTGCGCTCGTTCCAGTAAATAAAACAAGTGCGGCGAATTGGAAGAACGCGGCGTATTCCAAGAAATTGCAAATCAAAGCAGGGAGGGAGAATTAGTTCAAT	AGATGGAGCTGGAATAATCAATGGTTAGGGCTTATCTATATCGAAATCTAGGTAATTTATCAAATGTAGGTCTTTCTCAAAACCGGGTGAATTACTTAAATTTAAGCTTTAAAAACAGTTTTCAGTTGCATATGTATATCTGCGGCTACAATGAAGGTAAAAGGTAAACCGATATTCTTATTGCCCTATTCCGTAGACGA

 

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