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CRIMIC CR02239

Gene mmd

CG42252

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	X:15946936..15973159	14A1-14A4	FBti0213520	91492

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCAAAACTCTCTCTCTGGTCCTC	CCAATTCGACGGACAAGCTCTTGA

Images

Construct Design Sequences

sgRNA sequence	GCTTTCCTGTGCAAAATAAGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGTAGAGTTTTAGAATTCCTACGCTTTACCGTGGCATACGAAATTCGGCAAAGCGTTTGGATTCTGAAACCCTACCGATCCATTATCATGTTAAAAAAAAACAATACAAACACTAATAGGTTAAGGCAATTATTGCATTAATGAAGTTTTAAGTGCTTTACGGCATCACCAAGTTGATTGCTTGCTTTCCTGTGCAAAAT	AAGAGGGGGCACATTTCCGGCCTTTTTGTCGCATAAATTAATGGAAATATCTGCTAGATAGATCGTGTTTATTCAAACAAGGCAGCAAATAATTTTCACTTTATTTCCAATTCACTTTAGATACCTTAATTAACTTAGCGAATCAATCAAAGAGCTAAATATATGAAAATTACCTTTTTGCAGAAGCAGATTACACACTA

 

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