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CRIMIC CR02253

Gene yellow-h

CG1629

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	4:227922..230428	102B1	FBti0213521	91493

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGCATGCTATCGTATCGGATGTATGG	GGTTTCGATGTATCCCAGCATCCA

Images

Construct Design Sequences

sgRNA sequence	TACTTGATCAGTCTTACTTAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCGTTCTTTATTTTCATCCAATGGCAAGTTTCAAGGTGAGTATAAGGAGTATATAAATAAAGACAAGAAAATGGCTGGGAATTGATTTGATATTGATTTTAAAAAATGCCTTTCTTAACATTGTCTTTATTGTCTAAATAATGGCTCGCCGACTTACTTTTAAAAACCCCTCCTACAGCTTACTACTTGATCAGTCTTAC	TTAAGGTACTTAAATAAATCTAAATCTTAAGTAAGCTAATTATCTGCAGCTTGTAGGGTCCGAAAAGGCTTATTTCTGTTTTATTTTTATTAACCAAACTAGCAATCCCTTATACTCTACGAGTAACTGATATAATAATAATAATAATTGTGATTATAATACTTTCTCATTTAGGAGTTCATGGTGCCTATGAATATCTT

 

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