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CRIMIC CR02254

Gene Fatp3

CG3394

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2R:24294097..24298111	60C6		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	TGGTGCGAGACATACTCGGTTACT	CTGACTGAGTGGTCTGTAGATACC

Images

Construct Design Sequences

sgRNA sequence	CCCATGTCGCTCTTAGTCACTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GAGTTTCAGATATTTCCGATTTCTTTTTATTTAAGTTTGCATCATAAATAATTGATACCTTATCATCAAATGGTGGTCAGCAAGAAAGAATGGAATCTGGCTTTAATTACCGTTTAATAGCAATCTGAAATGTGTTTATAGATCGCATATATAACTATTGTTTCAGTGTAGCTGAGGGAACTACCCATGTCGCTCTTAGT	CACTGGGTTTCTGGGTTGTGCCATGTAGATAACAAACCACCTGAAACCACAAGCCGCAAACCTTGATTTTCACTATCGGCCCGCATATCTTTGCAGATCCCCCATGTGGAGGGCAAGGCGGGAATGGCGGCGATTGTGGATCCGGAGCGCAAGGTGGACATGGATTACCTGTCCGTGGTGCTGCGGGGCAGTTTGCCTCC

 

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