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CRIMIC CR02257

Gene ey

CG1464

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	4:697689..721173	102D4-102D5	FBti0216174	92659

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGCGGTCAATAGAGTCTGCGTTT	GGAGGTGGAATCGAATACGGAGAT

Images

Construct Design Sequences

sgRNA sequence	AAGGGTAAACCTGTCAACCACGG
sgRNA2 sequence

 

left_200bp	right_200bp
TAATTGAATCTCGATGTGGTACTAGTAAATTGGCTAAACAGGTATCATTTCAATAACAGTACATATTCTCGCCCAAAGCTTGCCCAAGAGCATGTGTGTCAAAACAGTCAATGCTCGACCTCCCCCGAACCACTTCCCAAAGCTTCCACTTTTGATCGAGCATAGCTGTGGGGCAACCACTGCCGTTACCACCGCCGTGG	TTGACAGGTTTACCCTTAACCGTGTAGTTTGCTTTAATGCGATTTAGAGACTCATTTTACAGGCGATGACACTTCATTAAACGACAAACCGTGAGGGTGGTTGTGGTTAGGGATGGGTTCACGGACAGGCCTAGAATGGTTGGATGCTAGATTGGATGCTGGGAGTCTTAGGGGTGCATAAATAGATTACAGAAATACAT

 

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