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CRIMIC CR02258

Gene nAChRalpha3

CG2302

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	X:8276200..8367169	7E1-7E4	FBti0213522	91494

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCGCTGCTCGGCAACATGAAAGT	GGATGTGTAAATCAGGCTGGAGTC

Images

Construct Design Sequences

sgRNA sequence	GGCATAACGCACGCGATGGATGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACGGCAAAATGGCAAAATGGTTTGCAATCCAAATGTGTTGACAAATTTTTGATTGGAGATTTTCGGGTAGCGGGCTGCGTTTTTGGGTGTTTCGCATTGGGGGCGAAATGCTCATTTAATAGATAATGCCCGTGATTGATGTCGCAGGCCTTAACCCCATTTGGACCAACAACAAAGTTGATGGGCATAACGCACGCGAT	GGATGGTAGATGATGGCGGTGGTACATGTACACGTATCGTGAATTGGATTAGGGATTCATTCCGCTACAGAATCCAACAGAGACTGATTACCGGGCGACAAACAATGAGGATAATTTTTGGGGCATACAATCGTTTCGTTTGTTCTTTGGCACAAAGTTAATTGGGAACTGTGACGTTTAGGAGATGTCAGTTGATTTAC

 

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