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CRIMIC CR02259

Gene nAChRbeta2

CG6798

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	3R:24509228..24515106	96A5	FBti0213523	91495

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCATCTCCGTTGAAGCATCTGTCA	TACCTGCTTCACCCACAGATTGGT

Images

Construct Design Sequences

sgRNA sequence	GGGATACAGCATCAGGGTATCGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTCCTAGCAAAATGTGTTAAGTTTAGTTAAAAACCTTCAATTATAAGCAATAAGTAGCACAAAAAGCTAGAGAAAACAGCAACAAAATGTGGCATTGGAGTTTGTTGTGTGTTTTCCTACTTGTTCCGTTGGCGAATTCCACGGGTAAGCTGCGAAAACTTGTGCGTCAGTCCGAGAAAAATCCTGTGAAAATTCCGATA	CCCTGATGCTGTATCCCAAGTATTTGACAGATGTCAGGAAGCTGCGCCCACAAGAGAGCAAGAGAGCGGCGTCTGCGCAGCAATTTGTTTTGGGAAATTTTCCATTTGTTTGGGAATTTAATTTGGCATTTTTCTCTGTTTTTCCCCTGAATTACCACCACTTTCCTAGCTCCAATAAGTTTTGAGGCAAATCCGGACAC

 

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