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CRIMIC CR02260

Gene side-II

CG42313

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2L:14907489..14972300	35B7	FBti0217649

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	TGCAGCCAAGTTTGGTGACTCGTA	CCCACTATCTTCGCTTCTGTTGGA

Images

Construct Design Sequences

sgRNA sequence	AGGTTGAGTCCTATTCTGCTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCTAAAAATGCAAAAATCAATATATCTGCAACTGAAGAAAAAAAACCCACTCAAAAACGTGCAACATTTACAACAAAATTTTATATTGTACAACGGATCACACACATAACTATTTTGCATAAAATAAAATTTGTCGTTCTGGGCTTTACGTATTTTTGGTGTGTGTGGGTGTTTTATTTTATTTTTTTTTTTACCCAAGC	AGAATAGGACTCAACCTGGCACTGATATACACAAAGCACGTATGTGGAATGTTTCGGTTGCCATTGTCCTTCCGTATGTCCTTTTGTTGCTCGTTTGCTTGTCGCCCGGAGACAAAAGCTAAGAGGAATCTCTGTCAGTCAGCCGTTTTCCATTTCTCTTTCGAAACACACACATTGACCCAAATTTAAACAATTGAATC

 

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