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CRIMIC CR02261

Gene ATPsynbeta

CG11154

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	4:1031484..1034917	102F6	FBti0213524	91496

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCAGACATTTGCGTGTACGTC	CGGTTGTCTACTTCCAAGGCATTC

Images

Construct Design Sequences

sgRNA sequence	TATATTGCCAGCGTATGAAAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAAAATTGTCGATATTGATGCCCAAATTGTTACTGTTTAAGTTTTACGATGATGTATTCTACTAGTATCACTTTCGTTAAGTATAACATAAATCTGTATATATTGGTATCTAGTGATAGGAAAATCAATAAACTGATAGTACTTTTTATTAAAAAATGATACCAATTAGCTTTACATATGGGGTATTAAGAAAACCTTTT	CATACGCTGGCAATATAAATTAGCAAACTTTTCCGATCCCATAAATTTTATTCGATGATCAGGATCATCAGCCGAATGTGTTTTATTCATTCATTAAACAAATCAAGCATCTAATACAATGTTAGATACATATATATTTTTTTGCAAGCACCTGCTGCGGTGGCCTTCGACAAGTCCTGATTTTTTATTTACAATTATTC

 

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