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CRIMIC CR02262

Gene myo

CG1838

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	4:690686..696055	102D4	FBti0214876	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAATTCTAAGTCGATCCAGATGCCC	GAGGTTGATCACTGCAAGTGGCTTAG

Images

Construct Design Sequences

sgRNA sequence	GAGTAGGTATAGATAAAGGGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TAGATTTGAATAAAAATCAAGCTAAAAAGTCGGACATTCCTATTAATACCAACGATGAGGAGTACGAGAGTATTTTGTCTCACATCAGCAGTATATACATATTTCCCGAGGGTAAGTAAACAGCATTCTTAAAGTAGATATAGATATAGGAAGATATTGAATAAGAGAAGATATTATATATAAGAGTAGGTATAGATAAA	GGGAGGAAAAGACAAGGAGAGCTTAAGAGAAGTAAAAAGCATATATAACGTCACTAAAATCTGTGTAAATATATTTCTAGACTTTTTGTAAGTAAGAAGCATAATCATTTATCTTATATTTATATCTTTTTTGGGCCCGATTTGGTTATAGGTTTAGATTTCGAACTTTTTTCCTTTAATAAGAGAAAGCAAGCAAAAAT

 

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