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CRIMIC CR02270

Gene poe

CG14472

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	2L:8054322..8071624	28D11-28E1	FBti0213526	91498

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TAAAGCTGTGTGGTATCCCTGCTC	CGTGCAACTGACAAATCTGCGTCT

Images

Construct Design Sequences

sgRNA sequence	AAAATTAAATATGGCCGCTCCGG
sgRNA2 sequence

 

left_200bp	right_200bp
GAACGGATTGGAATTCCGTTGTTAAAGCCCTGATACTTAACCGTACAGGAGCCTTAAACAAGAACGAAGTTGTTAATTTGCTCAAAGCTATCACGCGATGGTAAGTACAAACGATCATCTAGAACTTTTCGCCTACCGAATGGCGTAGTGAAAAATTTTCTGCTAGTGCAAAAAGTGACTTGCAAAATTAAATATGGCCG	CTCCGGTGACGAAATAGCAAAAAGCTTTGAAAATTCTTTAATATCGAGAACTAACAACGCTCTATTCCGATATTTGCATAGCGAACATGACTTTTTCGAGGAGGAAAGCAATTTCACGCAGTTTTACACGGCCTTTGCGGCCCTGGCGGCGGACAAACTTATGCAAATCAAGACAAGTAAGTGCACCCTAATGATTTTTC

 

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