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CRIMIC CR02280

Gene He

CG31770

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2L:13972096..13972947	34E5	FBti0214877	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGGGTACTAATCTCGCTCCGCAGAA	TGGGCTTGCATTGCTCTTTCACAG

Images

Construct Design Sequences

sgRNA sequence	GCAATTTGACCCTTTGTCATGGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATTTTTTGGCACTCGGCGGAGCAGTTCACACTAAGTTTACATAAGTCACTAATATGCATAATTTTCAACTGACCGTCGTTTTCCTGGCACTGTTCACTTTTACCAGTTCCTTTGGATACCGTTCTGAACATCCGCACAGAAAACCAATACATGTACGTACTTTTTCTGTCTTTTAAAATCAATAACATTAAATACCCATG	ACAAAGGGTCAAATTGCATATGCAAATGACATACAAATATGTTAGTCTAAATATTACATATTTATAGGTATTTAATTAAAAAAATTTAATTTTAAAAACCAAGAATTATCATAATTTTTTGTTTTTGTCAGAGCAACCGTCCATCTCCAACTACTACAATGACAACAGATTCAACTTCAACTAGCTTTATGACAACAGAT

 

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