( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 28
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#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 29
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#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 30
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1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 31
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1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 32
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1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 33
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1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 34
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1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 35
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#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 36
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1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 37
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#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 38
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#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 39
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#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 40
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#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 41
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#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

( ! ) Warning: Trying to access array offset on null in C:\wamp64\www\pscreen\crimic\info.php on line 44
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#	Time	Memory	Function	Location
1	0.0001	358192	{main}( )	...\info.php:0

CR02283

CRIMIC CR02283

Gene TbCMF46

CG13125

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	:..			Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
		GAATCGACCATCTCTGGATGATGC	TGCATTCCATCCAAGTGCTCCACA

Images

Construct Design Sequences

sgRNA sequence	CGGAAACACTAAATTAAAAATGG
sgRNA2 sequence

left_200bp	right_200bp
AATCTAGAAAAACTGGTCAAGCTGGAGAAGCTATCCCTGTTTAGCAATCGCATCAGGAAAATCGAAAACATACATACTTTGCAGAATCTTGTTATCCTTAGCATTGGAAACAATCTCATCGATACGGTAGAAGGCGTAAGGTTTTTTTCTATTTCATTTCGATAATACTATAAATTTCTTTTTCGGAAACACTAAATTAA	AAATGGCCCCTTTTAGATTGTGTCGAAATTAAAAAGTTCATAAATTAAAGTATGGTTTTTCATCCAGATCGAACGATTACGGTTTGTGAGCAGCCTGAAAGTCCTTAATTTGGAGGGCAATCCCATAGCCAAGCAGCCAGATTTCCCATTGTCCCTCTATGTGATTGCCATTCTACCCCAGCTGAACTACTACGAATACG