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CRIMIC CR02287

Gene Ns2

CG6501

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2R:17565779..17568456	54C8-54C9	FBti0213530	91502

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACGTGTGGTTTGGTTTGATCGTG	TGACAGCCTTGCCAATCTCATCCT

Images

Construct Design Sequences

sgRNA sequence	TTACCATCACTTATAGACATAGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAGCGGTCTCAAGGGAGTCGCCCACCCCCGCACTAAGGGCACCATCAAGCGGCTTCAGATGTACCGTAACTTCAAGGCCAAGCGCGATCGCACTGGAAAGATCCTCACGCCCGCCCCCTTCCAGGTGAGATTTAGCTTTACAACATGTATCTTCGATAATTCTTATCTTTGGATTCATAAGATTTACCATCACTTATAGA	CATAGGAAGGATTTATTGCAACATGAATATCCAATAACCATTGTTTAGTTTTCAAAATTATCATCTACTTCACAAAGGTGCAATTAAAACGATCGACTCAAAATTAAAAGCATTAAAAGATAAAAAGATTTCATAAGTATATTTTGTTACGACTTATTATCCGTATAATAATTTAATTACAAATCTTATGTTTTCATTCT

 

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