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CRIMIC CR02298

Gene Cul3

CG42616

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2L:15265245..15272024	35C5	FBti0213533	91505

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGTCCGCTCAGGGATTAGCTTCT	CGCTGCTGCACATAAACTCTATCC

Images

Construct Design Sequences

sgRNA sequence	AAATGCATAAGAGTGTAAGAGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCTCTGGGCCATCAACCTCAGCTTCAGCGAGCGCAGAAAGTACAGAAAAACGCTTTAAGGAAATAGCACGGAAGTACCCACTCTGGCTACCGGAGTACAAGCGAAGGGCATTCAATGTTAGCCTACCATCTGATAAAAACCAAACAGGGATTTCAACTTTTAGTTAACTTACTAACAAGCGAAAGTCTTAATATCCCTCT	TACACTCTTATGCATTTATAAGTTTTAATCCCTGAGAAATAAGTTCTTGACTATCCAAATTAATAATCTTATATTTCTCTTCCAGGCCTCCATGGACGAGAAGTACGTGGAGACCATTTGGGCCAGCTTAAAGAATGCTATCCAGGAGATACAGAAAAAGAACAACTCCGGACTATCGTTTGAACAACTCTACCGGAATG

 

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