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CRIMIC CR02311

Gene Wdr59

CG4705

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2L:11091444..11095096	32D2-32D3	FBti0213540	91512

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTGGCTGGCAGTTGCTTTCGAAG	CCTTAGCTTGGCTAGTAAGTGGTC

Images

Construct Design Sequences

sgRNA sequence	AACCAAACGATGTATGGATTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACGGACGCCCAAATGCGAGCCCTGCAAGCGACCCGTACTCTTCTGCGTCCTCTGCCGTTTGCCTGTGAAAGGAGCAGCCAATGCGTGTCTGGCTTGTGGCCATGGCGGTCACATTGATCACATGATGCAGTGGTTTGAGGTTGGTATCTATTTGAGTTTGATTCGACTGGTTTCTTTTTCACGAACCAAACGATGTATGG	ATTTGGATTTATGGATTTATGGATATAAATATATTCATGTTTGCTATTTTCTTGTTTAACTTTCAGAAACACAATGTCTGTGCTACGTGTGGCTGCAAGTGCTTGGAGCGCACCTCAGAGCTGCTAGCCCTACTCAGCTGATTCAGACCTTGAGTGATGTGCATTCCTTTCAAAAATAATCTTACTTAACTTTCTTTCGT

 

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