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CRIMIC CR02315

Gene rhi

CG10683

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2R:17628491..17633516	54D2	FBti0213541	91513

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTAGGTCATCCGATGGTGGTCAT	GCACAATGATCCTCAAGCTCTCGA

Images

Construct Design Sequences

sgRNA sequence	ATTACTTAAGCCTGATAGGTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATTGGCTTGGATTTATCTGGCAGTGATTCCATGGATTCCGAAGTGGAGAGCGTAAGAACTGTTCTTTCCAATTAAGAAAATCAAACCAAAGCTAGTAAAAATATGTTTATAATCATTTAAACCATTAAAAAAAAACCTAAATGTAAAAGCGTTTGAATAGAAACCTTTAAAAGTTAATCAGCTTAAGCCTAATTCCTACC	TATCAGGCTTAAGTAATGAATCAAAAGTTTCTTTAGCAATGATTTGATTTTCTTGTCCATTAAAACAACACACCTAAGAAAAATGAATTGTATTGGAACAGATGCGCCGCTGTCCGCGCCGCAAGCGTAAAAAAACTTACCCGGATTGGAAATTCCCGGAGATGACGAAACCCTTCGGTGTGAATCGCGGACTGGATCTG

 

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