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CRIMIC CR02327

Gene CG34205

CG34205

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2R:22130112..22131299	2-98	FBti0214879	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	TCTAGTCAAGATCTGTCCGCTCGA	TCTTACCGAGCTGAAGTGCGAGTA

Images

Construct Design Sequences

sgRNA sequence	ATCTAGTGAATATAAGATCGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGGGTATAAAAGTAACGCAATGCACACGTCCACTTACAGTTGCAATTCTCCCTCCAAGCCAAGTGATTCGTTATCCAGCGCTAGCATGAAACTTCTGGTGAGTGGAAAGGGTTGGAAAGGATGCGGCCTAGGATCAGTATTATCACTTTAAGCCAATAATACTGACTGCAAAGGATATCTTCATACAAGGATTACCTCGA	TCTTATATTCACTAGATCAAAAAGATTAACATCAAAGTTTGGTTTTCCATTTACCTCGCCAGATCCTTACCTCTCTGCTGGCTGTGGCCACTGCTGCTCCTGGACTCCTTGATTATGGACACTCGGCTCCGGACTACAGCTACGCCCATGCCGCTCCTGCCATAACATATGCAGCTGCAGCTCCAGTCATCAAGTCCTAC

 

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