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CRIMIC CR02330

Gene GMF

CG5869

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2L:16301656..16302954	35F1	FBti0214881	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCACTGCAAGGGACAATGGAGTG	TGAACGGTCTCACTGCAGGAACAA

Images

Construct Design Sequences

sgRNA sequence	AAACATGACTAATAGAGTGTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAACAAAACTCAGAATAAACGACAGTATTTAAACGATTCCTTAAGGACAAGTGATATTGCATTCCTACAATTAACTGCCAAAAATTCGGTAGTGCAGCACACTTTACAGATCGCATCCCTAACTTCATAGCTAAAGTTATGGTAGGAAGTGTATATAGGGTCGGAAATCAATTACCAAAATGAAAACATGACTAATAGAG	TGTGGGCGTGTTTTTGTTTTTGTTTTTCAATCGACGATTGTTGTTAGCAGATAACTTTGGTGATTCCAATTCATTAATCAATTATATCGATTTCATACAGAGTGATAACCAGATATGCGACATAAGCAACGAAGTTCTGGAGGAATTGAAGAAGTTTCGATTCAGCAAGAGCAAAAATAATGCGGCTTTGATATGTAAGC

 

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