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CRIMIC CR02335

Gene Fis1

CG17510

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2R:5603587..5604834	41F5	FBti0214882	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATCTTGATGTGCACCTTTCGATG	ATTCTGTCTGGTCCGAAGTCGGTA

Images

Construct Design Sequences

sgRNA sequence	TTAGTGTATGTACATTAGTGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GAGTCAAATGATCAAGTTCGCTCCCTAGAGGTTAGTAAGGTCACTAAGGAAAGCAGCAATTTCTATATAATCTCCAATAGATTCAAATAATTTTTTGATCCAGAAAATTTTTATGACAACCAAAATCCCATGATCACAAAAGATGTGTCGTGTTAAATGTACATATATAATAATGAAAAACATATTCATGTGATCCTCAC	TAATGTACATACACTAAAATTCAATGTACGAATAATTACTAAATAACTTTACATATTTCAGGAATATATTAAAAAAGAAATCGATAAGGAAGTGGCAAAGGGTATGGTGGTTGCAGGCGGAGCAGCTTTAGTACTTGGCGGAATATTAGGACTTGGCATTGCTATGGCTAGAAAGTAATATTTTATACCAACACGTTTTT

 

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