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CRIMIC CR02343

Gene ACXE

CG17178

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2L:12925611..12929823	34A2	FBti0217650

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCAAAGGAACGCATGTGGGAACCT	ATGAAGTGGATCGGCAGGAACATG

Images

Construct Design Sequences

sgRNA sequence	AAGAAAAACATCTCATTTAATGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGCAGAGTTGCGTTTGGAGAGCGAGTTTCGACTGTATCGGATACGGCTTTGGAAGAGTTACCTACTCACTTTTTTTATGCTGCATATATTCGTTACCTCCGTGCACTGTGCCCTACTTTTAGCAACGATTGAGGTAAGAACCGCCTAACCTAGATACAATTTTAGACCAACAGTTGCACAACATATTTAAAAAACCATTA	AATGAGATGTTTTTCTTCCATCTTGTAATTCTTTTATATATATGTTTTATTCTCGTTATTGTACCTTTTGTAGCGCCGATCAATTATTTATTTCGATGTAGCCCTTTCCATAGGATGCGCATTGGTCCTTATACTTGTGCTGAGTGTTAACTTCTGCGATGAATTTATTGCGAAACACACGTGGTACATGTATGCCAGCT

 

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